ELISA/assay

Rat liver type fatty acid binding protein, L-FABP ELISA Kit

MBS700219

48-Strip-Wells

565 USD

ELISA Kit

Rat liver type fatty acid binding protein, L-FABP ELISA Kit

fatty acid binding protein 1, liver

Rat liver type fatty acid binding protein (L-FABP) ELISA Kit; FABPL; L-FABP; Fatty acid-binding protein; liver; fatty acid binding protein 1; liver

fatty acid-binding protein, liver; Fatty acid-binding protein, liver; fatty acid-binding protein, liver; Z-protein; squalene- and sterol-carrier protein; liver-type fatty acid-binding protein; fatty acid binding protein 1, liver; Fatty acid-binding protein 1; Liver-type fatty acid-binding protein; L-FABP; Squalene- and sterol-carrier protein; SCP; Z-protein; p14

FABP1

Fabp1; Fabp1; SCP; p14; SCP.; Fabplg; L-FABP; L-FABP; SCP

FABPL_RAT

Rat

127

This assay has high sensitivity and excellent specificity for detection of rat L-FABP. No significant cross-reactivity or interference between rat L-FABP and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 1.56 ng/ml-100 ng/ml. Sensitivity: The minimum detectable dose of rat L-FABP is typically less than 0.39 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for L-FABP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any L-FABP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for L-FABP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of L-FABP bound in the initial step. The color development is stopped and the intensity of the color is measured.

6978825

NP_036688.1

NM_012556.2

P02692

14,273 Da

Fat Digestion And Absorption Pathway (194376); Fat Digestion And Absorption Pathway (194324); Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway (1010691); Metabolism Pathway (1010675); Metabolism Of Lipids And Lipoproteins Pathway (1010682); PPAR Signaling Pathway (83434); PPAR Signaling Pathway (450); PPARA Activates Gene Expression Pathway (1010711); Regulation Of Lipid Metabolism By Peroxisome Proliferator-activated Receptor Alpha (PPARalpha) Pathway (1010710); Selenium Metabolism Selenoproteins Pathway (219789)

binds oleic acid and other hydrophobic ligands; may play a role in hepatocyte cell proliferation; may transport activated chemical carcinogens [RGD, Feb 2006]

Function: Binds free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules in the cytoplasm. May be involved in intracellular lipid transport.

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150