Rat ornithine carbamoyl transferase, OCT ELISA Kit | MyBioSource MBS700217 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat ornithine carbamoyl transferase, OCT ELISA Kit

catalog :

MBS700217

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS700217

products type :

ELISA Kit

products full name :

Rat ornithine carbamoyl transferase, OCT ELISA Kit

products short name :

ornithine carbamoyltransferase

products name syn :

Rat ornithine carbamoyl transferase; OCT ELISA Kit; MGC129967; MGC129968; MGC138856; OCTD; ornithine transcarbamylase; ornithine carbamoyltransferase

other names :

ornithine carbamoyltransferase, mitochondrial; Ornithine carbamoyltransferase, mitochondrial; ornithine carbamoyltransferase, mitochondrial; OTCase; ornithine transcarbamylase; ornithine carbamoyltransferase; Ornithine transcarbamylase; OTCase

products gene name :

OTC

other gene names :

Otc; Otc; OTCase

uniprot entry name :

OTC_RAT

reactivity :

Rat

sequence length :

354

specificity :

This assay has high sensitivity and excellent specificity for detection of rat OCT. No significant cross-reactivity or interference between rat OCT and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, cell culture supernates. Assay Type: Sandwich. Detection Range: 1.56 mU/ml -100 mU/ml. Sensitivity: The minimum detectable dose of rat OCT is typically less than 0.39 mU/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for OCT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any OCT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for OCT is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of OCT bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

6981312

ncbi acc num :

NP_037210.1

ncbi gb acc num :

NM_013078.1

uniprot acc num :

P00481

ncbi mol weight :

39,886 Da

ncbi pathways :

Arginine And Proline Metabolism Pathway (83349); Arginine And Proline Metabolism Pathway (323); Biosynthesis Of Amino Acids Pathway (791250); Biosynthesis Of Amino Acids Pathway (795174); Metabolism Pathway (1010675); Metabolism Of Amino Acids And Derivatives Pathway (1010820); PI3K-PEPCK-VTN Pathway (755432); Urea Cycle Pathway (434668); Urea Cycle Pathway (1010829); Urea Cycle Pathway (468222)

ncbi summary :

catalyzes the conversion of L-ornithine to L-citrulline [RGD, Feb 2006]

uniprot summary :

OTC: Defects in OTC are the cause of ornithine carbamoyltransferase deficiency (OTCD). OTCD is an X- linked disorder of the urea cycle which causes a form of hyperammonemia. Mutations with no residual enzyme activity are always expressed in hemizygote males by a very severe neonatal hyperammonemic coma that generally proves to be fatal. Heterozygous females are either asymptomatic or express orotic aciduria spontaneously or after protein intake. The disorder is treatable with supplemental dietary arginine and low protein diet. The arbitrary classification of patients into the neonatal group (clinical hyperammonemia in the first few days of life) and late onset (clinical presentation after the neonatal period) has been used to differentiate severe from mild forms. Belongs to the ATCase/OTCase family. Protein type: Mitochondrial; Lipid-binding; Transferase; EC 2.1.3.3; Amino Acid Metabolism - arginine and proline. Cellular Component: mitochondrion; mitochondrial matrix; mitochondrial inner membrane; cytoplasm. Molecular Function: amino acid binding; ornithine carbamoyltransferase activity; phospholipid binding; phosphate binding. Biological Process: response to drug; response to nutrient levels; citrulline biosynthetic process; response to zinc ion; ornithine metabolic process; midgut development; arginine biosynthetic process via ornithine; liver development; ornithine catabolic process; anion homeostasis; response to insulin stimulus; urea cycle

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

2750

size4 :

10x96-Strip-Wells

price4 :

5150

more info or order :

MyBioSource product webpage