ELISA/assay

Rat matrix metalloproteinase 9/Gelatinase B, MMP-9 ELISA Kit

MBS700216

48-Strip-Wells

465 USD

ELISA Kit

Rat matrix metalloproteinase 9/Gelatinase B, MMP-9 ELISA Kit

matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)

Rat matrix metalloproteinase 9/Gelatinase B; MMP-9 ELISA Kit; CLG4B; GELB; MANDP2; MMP-9; macrophage gelatinase; matrix metalloproteinase 9; matrix metalloproteinase 9 (gelatinase B; 92kDa gelatinase; 92kDa type IV collagenase) ; type V collagenase; matrix metallopeptidase 9 (gelatinase B; 92kDa gelatinase; 92kDa type IV collagenase)

matrix metalloproteinase-9; Matrix metalloproteinase-9; matrix metalloproteinase-9; GELB; MMP-9; gelatinase B; 92 kDa gelatinase; 92 kDa type IV collagenase; matrix metalloproteinase 9; 92 kDa gelatinase; 92 kDa type IV collagenase; Gelatinase B; GELB

MMP9

MMP9; MMP9; MMP-9; GELB

MMP9_RABIT

Rat

707

This assay has high sensitivity and excellent specificity for detection of Rat MMP9. No significant cross-reactivity or interference between Rat MMP9 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml-1000 pg/ml. Sensitivity: 3.9 pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Developmental Protein. Protein Biological Process 3: Collagen degradation

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MMP-9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MMP-9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MMP-9 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMP-9 bound in the initial step. The color development is stopped and the intensity of the color is measured.

126723483

NP_001075672.1

NM_001082203.1

P41246

78,308 Da

Activation Of Matrix Metalloproteinases Pathway (576264); Bladder Cancer Pathway (83115); Bladder Cancer Pathway (527); CXCR4-mediated Signaling Events Pathway (137910); Degradation Of The Extracellular Matrix Pathway (576263); Endochondral Ossification Pathway (198812); Extracellular Matrix Organization Pathway (576262); FGF Signaling Pathway (137989); IL-3 Signaling Pathway (198881); LPA Receptor Mediated Events Pathway (137928)

Function: Could play a role in bone osteoclastic resorption. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments . Catalytic activity: Cleavage of gelatin types I and V and collagen types IV and V. Cofactor: Binds 2 zinc ions per subunit

48-Strip-Wells

465 USD

96-Strip-Wells

655

5x96-Strip-Wells

2455

10x96-Strip-Wells

4595