product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Rat matrix metalloproteinase 1, MMP-1/Collagenase I ELISA Kit
catalog :
MBS700215
quantity :
48-Strip-Wells
price :
465 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS700215
products type :
ELISA Kit
products full name :
Rat matrix metalloproteinase 1, MMP-1/Collagenase I ELISA Kit
products short name :
matrix metallopeptidase 1 (interstitial collagenase)
products name syn :
Rat matrix metalloproteinase 1 (MMP-1/Collagenase I) ELISA kit; CLG; CLGN; fibroblast collagenase; matrix metalloprotease 1; matrix metalloproteinase 1; matrix metallopeptidase 1 (interstitial collagenase)
other names :
interstitial collagenase; Interstitial collagenase; interstitial collagenase; collagenase-1; matrix metalloproteinase-1; Matrix metalloproteinase-1; MMP-1
products gene name :
MMP1
other gene names :
MMP1; MMP1; MMP-1; MMP-1
uniprot entry name :
MMP1_RABIT
reactivity :
Rat
sequence length :
468
specificity :
This assay has high sensitivity and excellent specificity for detection of rat MMP-1. No significant cross-reactivity or interference between rat MMP-1 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, cell culture supernates, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 62.5 pg/ml -4000 pg/ml. Sensitivity: The minimum detectable dose of rat MMP-1 is typically less than 15.6 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MMP-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MMP-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MMP-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMP-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi gi num :
283837835
ncbi acc num :
NP_001164610.1
ncbi gb acc num :
NM_001171139.1
uniprot acc num :
P13943
ncbi mol weight :
53,740 Da
uniprot summary :
Function: Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. Catalytic activity: Cleavage of the triple helix of collagen at about three-quarters of the length of the molecule from the N-terminus, at 775-Gly- -Ile-776 in the alpha-1(I) chain. Cleaves synthetic substrates and alpha-macroglobulins at bonds where P1' is a hydrophobic residue. Cofactor: Binds 4 calcium ions per subunit . Enzyme regulation: Can be activated without removal of the activation peptide.
size1 :
48-Strip-Wells
price1 :
465 USD
size2 :
96-Strip-Wells
price2 :
655
size3 :
5x96-Strip-Wells
price3 :
2455
size4 :
10x96-Strip-Wells
price4 :
4595
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

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Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

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