Human Cystatin C, Cys-C ELISA Kit | MyBioSource MBS700210 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Cystatin C, Cys-C ELISA Kit

catalog :

MBS700210

quantity :

96-Strip-Wells

price :

680 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS700210

products type :

ELISA Kit

products full name :

Human Cystatin C, Cys-C ELISA Kit

products short name :

cystatin C

products name syn :

Human Cystatin C; Cys-C ELISA Kit; ARMD11; MGC117328; OTTHUMP00000164181; OTTHUMP00000164182; bA218C14.4 (cystatin C) ; cystatin 3; gamma-trace; neuroendocrine basic polypeptide; post-gamma-globulin; Human Cystatin C; Cys-C

other names :

cystatin-C; Cystatin-C; cystatin-C; cystatin 3; cystatin-3; gamma-trace; post-gamma-globulin; bA218C14.4 (cystatin C); neuroendocrine basic polypeptide; cystatin C; Cystatin-3; Gamma-trace; Neuroendocrine basic polypeptide; Post-gamma-globulin

products gene name :

CST3

other gene names :

CST3; CST3; ARMD11

uniprot entry name :

CYTC_HUMAN

reactivity :

Human

sequence length :

146

specificity :

This assay has high sensitivity and excellent specificity for detection of human Cys -C. No significant cross-reactivity or interference between human Cys -C and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, urine, saliva, tissue homogenates. Assay Type: Sandwich. Detection Range: 7.81 ng/ml -500 ng/ml. Sensitivity: The minimum detectable dose of human Cys -C is typically less than 5.824 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Priciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for Cys-C has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Cys-C present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Cys-C is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Cys-C bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

4503107

ncbi acc num :

NP_000090.1

ncbi gb acc num :

NM_000099.3

uniprot acc num :

P01034

ncbi mol weight :

15,799 Da

ncbi pathways :

Amyloids Pathway (366238); Disease Pathway (530764); Integrated Pancreatic Cancer Pathway (711360); Salivary Secretion Pathway (153376); Salivary Secretion Pathway (153352)

ncbi summary :

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they appear to provide protective functions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes the most abundant extracellular inhibitor of cysteine proteases, which is found in high concentrations in biological fluids and is expressed in virtually all organs of the body. A mutation in this gene has been associated with amyloid angiopathy. Expression of this protein in vascular wall smooth muscle cells is severely reduced in both atherosclerotic and aneurysmal aortic lesions, establishing its role in vascular disease. Alternative splicing results in multiple transcript variants encoding a single protein. [provided by RefSeq, Dec 2013]

uniprot summary :

Function: As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.

size1 :

96-Strip-Wells

price1 :

680 USD

size2 :

5x96-Strip-Wells

price2 :

2140

size3 :

10x96-Strip-Wells

price3 :

3990

more info or order :

MyBioSource product webpage