catalog number :
MBS700195
products type :
ELISA Kit
products full name :
Rat soluble interleukin-2 receptor, sIL-2Rbeta ELISA Kit
products short name :
interleukin 2 receptor, beta
products name syn :
Rat soluble interleukin-2 receptor; sIL-2Rbeta ELISA kit; RP5-1170K4.6; CD122; P70-75; CD122 antigen; high affinity IL-2 receptor beta subunit; interleukin 2 receptor beta; interleukin 2 receptor; beta
other names :
interleukin-2 receptor subunit beta; Interleukin-2 receptor subunit beta; interleukin-2 receptor subunit beta; IL-2RB; P70-75; IL-2R subunit beta; IL-2 receptor subunit beta; interleukin 2 receptor, beta chain; high affinity IL-2 receptor subunit beta; interleukin 2 receptor, beta; High affinity IL-2 receptor subunit beta; p70-75; CD_antigen: CD122
products gene name :
IL2RB
other gene names :
Il2rb; Il2rb; IL2RBC; IL-2 receptor subunit beta; IL-2R subunit beta; IL-2RB
uniprot entry name :
IL2RB_RAT
specificity :
This assay has high sensitivity and excellent specificity for detection of Rat IL2RB. No significant cross-reactivity or interference between Rat IL2RB and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Assay Type: Sandwich
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique.Antibody specific for sIL-2Rbeta has been pre-coated onto a microplate. Standardsand samples are pipetted into the wells and any sIL-2Rbeta present is bound by theimmobilized antibody. After removing any unbound substances, abiotin-conjugated antibody specific for sIL-2Rbeta is added to the wells. Afterwashing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells.Following a wash to remove any unbound avidin-enzyme reagent, a substratesolution is added to the wells and color develops in proportion to the amount ofsIL-2Rbeta bound in the initial step. The color development is stopped and theintensity of the color is measured.
ncbi acc num :
NP_037327.1
ncbi gb acc num :
NM_013195.1
ncbi mol weight :
60,657 Da
ncbi pathways :
Cytokine-cytokine Receptor Interaction Pathway (83443); Cytokine-cytokine Receptor Interaction Pathway (460); Endocytosis Pathway (102267); Endocytosis Pathway (102181); HTLV-I Infection Pathway (373894); HTLV-I Infection Pathway (373889); IL-2 Signaling Pathway (198476); IL-5 Signaling Pathway (198483); Inflammatory Response Pathway (198472); Jak-STAT Signaling Pathway (83469)
ncbi summary :
beta chain of receptor for interleukin 2 [RGD, Feb 2006]
uniprot summary :
Function: Receptor for interleukin-2. This beta subunit is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2.
size4 :
10x96-Strip-Wells