catalog number :
MBS700193
products type :
ELISA Kit
products full name :
Mouse Lipoxin A4, LXA4 ELISA Kit
products short name :
Lipoxin A4, LXA4
products name syn :
Mouse Lipoxin A4; LXA4 ELISA Kit; Lipoxin A4; LXA4
other names :
lipoxin A4 receptor; N-formyl peptide receptor 2; N-formyl peptide receptor 2; RFP; FMLP-R-I; LXA4 receptor; FMLP-related receptor I; formyl peptide receptor-like 1; lipoxin A4 receptor (formyl peptide receptor related); formyl peptide receptor 2; FMLP-related receptor I; FMLP-R-I; Formyl peptide receptor-like 1; HM63; Lipoxin A4 receptor; LXA4 receptor; RFP
products gene name :
LXA4
other gene names :
FPR2; FPR2; ALXR; HM63; FMLPX; FPR2A; FPRH1; FPRH2; FPRL1; LXA4R; FMLP-R-II; FPRH1; FPRL1; LXA4R; FMLP-R-I; LXA4 receptor
uniprot entry name :
FPR2_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse LXA4. No significant cross-reactivity or interference between mouse LXA4 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates, cell culture supernates. Assay Type: Sandwich. Detection Range: 62.5 pg/ml -400 0 pg/ml. Sensitivity: 15.6 pg/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LXA4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LXA4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LXA4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LXA4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
AAC13684.1
ncbi mol weight :
38,964 Da
ncbi pathways :
Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Formyl Peptide Receptors Bind Formyl Peptides And Many Other Ligands Pathway (161021); G Alpha (i) Signalling Events Pathway (119550); G Alpha (q) Signalling Events Pathway (106043); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); GPCRs, Class A Rhodopsin-like Pathway (198886); Gastrin-CREB Signalling Pathway Via PKC And MAPK (645295); Neuroactive Ligand-receptor Interaction Pathway (83053); Neuroactive Ligand-receptor Interaction Pathway (462)
uniprot summary :
Function: Low affinity receptor for N-formyl-methionyl peptides, which are powerful neutrophils chemotactic factors. Binding of FMLP to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. The activation of LXA4R could result in an anti-inflammatory outcome counteracting the actions of proinflammatory signals such as LTB4 (leukotriene B4).
size4 :
10x96-Strip-Wells