ELISA/assay

Human Aquaporin 4, AQP-4 ELISA Kit

MBS700190

48-Strip-Wells

565 USD

ELISA Kit

Human Aquaporin 4, AQP-4 ELISA Kit

aquaporin 4

Human Aquaporin 4; AQP-4 ELISA Kit; HMIWC2; MGC22454; MIWC; aquaporin type4; aquaporin-4; mercurial-insensitive water channel; aquaporin 4

aquaporin-4 isoform a; Aquaporin-4; aquaporin-4; WCH4; aquaporin type4; aquaporin 4 isoform delta4; mercurial-insensitive water channel; aquaporin 4; Mercurial-insensitive water channel; MIWC; WCH4

AQP4

AQP4; AQP4; MIWC; HMIWC2; AQP-4; MIWC

AQP4_HUMAN

Human

323

This assay has high sensitivity and excellent specificity for detection of Human AQP4. No significant cross-reactivity or interference between Human AQP4 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Detection Range: 0.156 ng/ml-10 ng/ml. Sensitivity: 0.039 ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Transport. Protein Biological Process 3: Transport

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated withan antibody specific to AQP-4. Standards or samples are thenadded to the appropriate microtiter plate wells with abiotin-conjugated antibody preparation specific for AQP-4 andAvidin conjugated to Horseradish Peroxidase (HRP) is added toeach microplate well and incubated. Then a TMB (3,3',5,5'tetramethyl-benzidine) substrate solution is added to each well.Only those wells that contain AQP-4, biotin-conjugated antibodyand enzyme-conjugated Avidin will exhibit a change in color. Theenzyme-substrate reaction is terminated by the addition of asulphuric acid solution and the color change is measuredspectrophotometrically at a wavelength of 450 nm +/- 2 nm. Theconcentration of AQP-4 in the samples is then determined bycomparing the O.D. of the samples to the standard curve.

4502181

NP_001641.1

NM_001650.4

P55087

34,830 Da

Aquaporin-mediated Transport Pathway (187187); Bile Secretion Pathway (193146); Bile Secretion Pathway (193095); Passive Transport By Aquaporins Pathway (187188); SIDS Susceptibility Pathways (198901); Spinal Cord Injury Pathway (739007); Transmembrane Transport Of Small Molecules Pathway (106572); Vasopressin Regulates Renal Water Homeostasis Via Aquaporins Pathway (187189); Vasopressin-regulated Water Reabsorption Pathway (143700); Vasopressin-regulated Water Reabsorption Pathway (143631)

This gene encodes a member of the aquaporin family of intrinsic membrane proteins that function as water-selective channels in the plasma membranes of many cells. The encoded protein is the predominant aquaporin found in brain. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Function: Forms a water-specific channel. Osmoreceptor which regulates body water balance and mediates water flow within the central nervous system.

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150