catalog number :
MBS700189
products type :
ELISA Kit
products full name :
Rat intercellular adhesion molecule 1, ICAM-1 ELISA Kit
products short name :
intercellular adhesion molecule 1
products name syn :
Rat intercellular adhesion molecule 1; ICAM-1 ELISA Kit; BB2; CD54; P3.58; cell surface glycoprotein P3.58; human rhinovirus receptor; intercellular adhesion molecule 1 (CD54); human rhinovirus receptor; Rat intercellular adhesion molecule 1; ICAM-1
other names :
intercellular adhesion molecule 1; Intercellular adhesion molecule 1; intercellular adhesion molecule 1; ICAM-1; intercellular adhesion molecule 1; CD_antigen: CD54
products gene name :
ICAM1
other gene names :
Icam1; Icam1; CD54; ICAM; Icam-1; ICAM-1
uniprot entry name :
ICAM1_RAT
specificity :
This assay has high sensitivity and excellent specificity for detection of Rat ICAM1. No significant cross-reactivity or interference between Rat ICAM1 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 78 pg/ml-5000 pg/ml. Sensitivity: 19.5 pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Cardiovascular
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ICAM-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ICAM-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ICAM-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ICAM-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_037099.1
ncbi gb acc num :
NM_012967.1
ncbi mol weight :
60,142 Da
ncbi pathways :
Adaptive Immune System Pathway (1010925); African Trypanosomiasis Pathway (194377); African Trypanosomiasis Pathway (194323); Cell Adhesion Molecules (CAMs) Pathway (83461); Cell Adhesion Molecules (CAMs) Pathway (480); Cytokine Signaling In Immune System Pathway (1011036); Epstein-Barr Virus Infection Pathway (585584); Epstein-Barr Virus Infection Pathway (587115); Extracellular Matrix Organization Pathway (1009923); HTLV-I Infection Pathway (373894)
ncbi summary :
cell adhesion molecule; ligand for leukocyte adhesion molecule LFA-1 [RGD, Feb 2006]
uniprot summary :
Function: ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation
size3 :
10x96-Strip-Wells