catalog number :
MBS700173
products type :
ELISA Kit
products full name :
Mouse acidic fibroblast growth factor, aFGF/FGF-1 ELISA Kit
products short name :
fibroblast growth factor 1 (acidic)
products name syn :
Mouse acidic fibroblast growth factor; aFGF/FGF-1 ELISA Kit; AFGF; ECGF; ECGF-beta; ECGFA; ECGFB; FGF-alpha; FGFA; GLIO703; HBGF1; OTTHUMP00000066031; endothelial cell growth factor; alpha; endothelial cell growth factor; beta; heparin-binding growth factor 1; fibroblast growth factor 1 (acidic)
other names :
fibroblast growth factor 1; Fibroblast growth factor 1; fibroblast growth factor 1; aFGF; HBGF-1; acidic fibroblast growth factor; heparin-binding growth factor 1; fibroblast growth factor 1 (acidic); fibroblast growth factor 1; Acidic fibroblast growth factor; aFGF; Heparin-binding growth factor 1; HBGF-1
products gene name :
FGF1
other gene names :
Fgf1; Fgf1; Fam; Fgfa; Dffrx; Fgf-1; Fgf-1; Fgfa; FGF-1; aFGF; HBGF-1
uniprot entry name :
FGF1_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of Mouse FGF1. No significant cross-reactivity or interference between Mouse FGF1 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 3.12 pg/ml-200 pg/ml. Sensitivity: 0.78 pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Angiogenesis. Protein Biological Process 3: Angiogenesis
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FGF-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FGF-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FGF-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FGF-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_034327.1
ncbi gb acc num :
NM_010197.3
ncbi mol weight :
17,418 Da
ncbi pathways :
Activated Point Mutants Of FGFR2 Pathway (1000637); Adaptive Immune System Pathway (971210); Constitutive PI3K/AKT Signaling In Cancer Pathway (1000676); DAP12 Interactions Pathway (971203); DAP12 Signaling Pathway (971214); Disease Pathway (970606); Downstream Signal Transduction Pathway (971469); Downstream Signaling Events Of B Cell Receptor (BCR) Pathway (1001193); Downstream Signaling Of Activated FGFR Pathway (971322); ESC Pluripotency Pathways (198374)
uniprot summary :
Function: Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro
size4 :
10x96-Strip-Wells