ELISA/assay

Human insulin-like growth factors 1, IGF-1 ELISA Kit

MBS700129

96 Strip Wells

645 USD

ELISA Kit

Human insulin-like growth factors 1, IGF-1 ELISA Kit

insulin-like growth factors 1, IGF-1

Human insulin-like growth factors 1; IGF-1 ELISA Kit; insulin-like growth factors 1; IGF-1

IGF-1

IGF-1

Human

71

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Sample Type: serum, plasma and tissue homogenates. Detection Range: 7.8 ng/ml-500ng/ml. Detection Wavelength: 450 nm. Sensitivity: 1.95 ng/ml

Sample Volume: 50-100ul

For Sample This immunoassay kit allows for the in vitro quantitative determination of human IGF-1 concentrations in serum, plasma . PRINCIPLE OF THE ASSAY The microtiter plate provided in this kit has been pre-coated with an antibody specific to IGF-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for IGF-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain IGF-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of IGF-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

1247519

CAA01954.1

Focal Adhesion Pathway 83067!!Focal Adhesion Pathway 478!!MAPK Signaling Pathway 83048!!MAPK Signaling Pathway 456!!Salmonella Infection Pathway 375172!!Salmonella Infection Pathway 375149

96 Strip Wells

645 USD