Human glutathione S-transferases, GSTpi ELISA Kit | MyBioSource MBS700126 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human glutathione S-transferases, GSTpi ELISA Kit

catalog :

MBS700126

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS700126

products type :

ELISA Kit

products full name :

Human glutathione S-transferases, GSTpi ELISA Kit

products short name :

glutathione S-transferase pi 1

products name syn :

Human glutathione S-transferases; GSTpi ELISA Kit; DFN7; FAEES3; GST3; GSTP; PI; deafness; X-linked 7; fatty acid ethyl ester synthase III; glutathione transferase; glutathione S-transferase pi 1

other names :

glutathione S-transferase P; Glutathione S-transferase P; glutathione S-transferase P; GSTP1-1; GST class-pi; deafness, X-linked 7; epididymis secretory protein Li 22; fatty acid ethyl ester synthase III; glutathione S-transferase pi 1; GST class-pi; GSTP1-1

products gene name :

GSTP1

other gene names :

GSTP1; GSTP1; PI; DFN7; GST3; GSTP; FAEES3; HEL-S-22; FAEES3; GST3

uniprot entry name :

GSTP1_HUMAN

reactivity :

Human

sequence length :

210

specificity :

This assay has high sensitivity and excellent specificity for detection of Human GSTP1. No significant cross-reactivity or interference between Human GSTP1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, cell supernates and tissue homogenates. Assay Type: Sandwich. Detection Range: 1.56 ng/ml-100 ng/ml. Sensitivity: 0.39 ng/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GSTP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GSTP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GSTP1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GSTP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

4504183

ncbi acc num :

NP_000843.1

ncbi gb acc num :

NM_000852.3

uniprot acc num :

P09211

ncbi mol weight :

23,356 Da

ncbi pathways :

4-hydroxy-2-nonenal Detoxification Pathway (703096); Arachidonate Epoxygenase / Epoxide Hydrolase Pathway (198838); Biological Oxidations Pathway (105698); Cellular Responses To Stress Pathway (645258); Chemical Carcinogenesis Pathway (673221); Chemical Carcinogenesis Pathway (673237); Detoxification Of Reactive Oxygen Species Pathway (941400); Diurnally Regulated Genes With Circadian Orthologs Pathway (198813); Drug Metabolism - Cytochrome P450 Pathway (83032); Drug Metabolism - Cytochrome P450 Pathway (427)

ncbi summary :

Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, the soluble GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. This GST family member is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases. [provided by RefSeq, Jul 2008]

uniprot summary :

Function: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Regulates negatively CDK5 activity via p25/p35 translocation to prevent neurodegeneration.1 PublicationManual assertion based on experiment in:Ref.25. Catalytic activity: RX + glutathione = HX + R-S-glutathione.3 PublicationsManual assertion based on experiment in:Ref.18

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

2750

size4 :

10x96-Strip-Wells

price4 :

5150

more info or order :

MyBioSource product webpage