product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Rat growth hormone releasing hormone, GHRH ELISA Kit
catalog :
MBS700124
quantity :
96 Strip Wells
price :
915 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS700124
products type :
ELISA Kit
products full name :
Rat growth hormone releasing hormone, GHRH ELISA Kit
products short name :
growth hormone releasing hormone
products name syn :
Rat growth hormone releasing hormone; GHRH ELISA Kit; GHRF; GRF; MGC119781; OTTHUMP00000030915; growth hormone releasing factor; sermorelin; somatocrinin; somatoliberin; growth hormone releasing hormone
other names :
somatoliberin; Somatoliberin; somatoliberin; GRF; growth hormone-releasing factor; growth hormone-releasing hormone; growth hormone releasing hormone; Growth hormone-releasing factor; GRF; Growth hormone-releasing hormone; GHRH
products gene name :
GHRH
other gene names :
Ghrh; Ghrh; GHRF; GRF; GHRH
uniprot entry name :
SLIB_RAT
reactivity :
Rat
sequence length :
104
specificity :
This assay has high sensitivity and excellent specificity for detection of rat GHRH. No significant cross-reactivity or interference between rat GHRH and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: For the quantitative determination of rat growth hormone releasing hormone (GHRH) concentrations in serum, plasma, tissue homogenates. Detection Range: 3.12 pg/ml -200 pg/ml.
other info2 :
Sensitivity: The minimum detectable dose of rat GHRH is typically less than 0.78 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: </b<This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GHRH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GHRH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GHRH is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GHRH bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi gi num :
13928782
ncbi acc num :
NP_113765.1
ncbi gb acc num :
NM_031577.1
uniprot acc num :
P09916
ncbi mol weight :
12,266 Da
ncbi pathways :
Class B/2 (Secretin Family Receptors) Pathway 1010189!!G Alpha (s) Signalling Events Pathway 1010194!!GPCR Downstream Signaling Pathway 1010193!!GPCR Ligand Binding Pathway 1010163!!Glucagon-type Ligand Receptors Pathway 1010191!!Signal Transduction Pathway 1010015!!Signaling By GPCR Pathway 1010162
ncbi summary :
stimulates growth hormone secretion; binds GHRH receptor and mediates adenylyl cyclase activation [RGD, Feb 2006]
uniprot summary :
Function: GRF is released by the hypothalamus and acts on the adenohypophyse to stimulate the secretion of growth hormone.
size :
96 Strip Wells
price :
915 USD
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

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