ELISA/assay

Rat aldehyde dehydrogenase, ALDH ELISA Kit

MBS700110

96 Strip Wells

850 USD

ELISA Kit

Rat aldehyde dehydrogenase, ALDH ELISA Kit

aldehyde dehydrogenase 2 family (mitochondrial)

Rat aldehyde dehydrogenase; ALDH ELISA Kit; ALDH-E2; ALDHI; ALDM; MGC1806; ALDH class 2; acetaldehyde dehydrogenase 2; liver mitochondrial ALDH; mitochondrial aldehyde dehydrogenase 2; nucleus-encoded mitochondrial aldehyde dehydrogenase 2; aldehyde dehydrogenase 2 family (mitochondrial)

aldehyde dehydrogenase, mitochondrial; Aldehyde dehydrogenase, mitochondrial; aldehyde dehydrogenase, mitochondrial; ALDH1; ALDH-E2; ALDH class 2; mitochondrial aldehyde dehydrogenase; aldehyde dehydrogenase 2, mitochondrial; aldehyde dehydrogenase 2 family (mitochondrial); ALDH class 2; ALDH-E2; ALDH1

ALDH2

Aldh2; Aldh2

ALDH2_RAT

Rat

519

This assay has high sensitivity and excellent specificity for detection of Rat ALDH2. No significant cross-reactivity or interference between Rat ALDH2 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, tissue homogenates. Detection Range: 0.312 ng/ml-20 ng/ml. Detection Wavelength: 450 nm. Sensitivity: 0.078 ng/ml

Sample Volume: 50-100ul

Introduction: Aldehyde dehydrogenases are a group of enzymes that catalyse the oxidation (dehydrogenation) of aldehydes. Mitochondrial Aldehyde Dehydrogenase is a polymorphic enzyme responsible for the oxidation of aldehydes to carboxylic acids, which leave the liver and are metabolized by the body's muscle and heart. There are three different classes of these enzymes in mammals: class 1 (low Km, cytosolic), class 2 (low Km, mitochondrial), and class 3 (high Km, such as those expressed in tumors, stomach and cornea). In all three classes constitutive and inducible forms exist. ALDH1 and ALDH2 are the most important enzymes for aldehyde oxidation, and both are tetrameric enzymes composed of 54kDA subunits. These enzymes are found in many tissues of the body, but are at the highest concentration in the liver. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ALDH. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for ALDH and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain ALDH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of ALDH in the samples is then determined by comparing the O.D. of the samples to the standard curve.

14192933

NP_115792.1

NM_032416.1

P11884

56,488 Da

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96 Strip Wells

850 USD