ELISA/assay

Mouse 8-Hydroxy-desoxyguanosine, 8-OHdG ELISA Kit

MBS700097

96 Strip Wells

895 USD

ELISA Kit

Mouse 8-Hydroxy-desoxyguanosine, 8-OHdG ELISA Kit

8-Hydroxy-desoxyguanosine, 8-OHdG

Mouse 8-Hydroxy-desoxyguanosine; 8-OHdG ELISA Kit; 8-Hydroxy-desoxyguanosine; 8-OHdG

8-OHdG

Mouse

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Sample Type: serum, plasma, tissue homogenates. Detection Range: 1.56 pg/ml-100 pg/ml. Detection Wavelength: 450 nm. Sensitivity: 0.39 pg/ml

Sample Volume: 50-100ul

Introduction: The role played by DNA in molecular biology is clearly recognized to be vital to life on this planet. 8-oxo-7,8-dihydro-2deoxyguanosine(=8-OHdG), is probably the most important product of "oxidative stress" in DNA. Its concentration in DNA is, in fact. a quantitative analysis of the degree of DNA damage that an organism has undergone. Due to the importance of 8-OHdG of nucleic acidg in mutagenesis, carcinogenesis and aging, numerous chemical and biological investigations have been made on this subject in the past time. Kuchino and co-workers have found that 8-OHdG residue in DNA is misreading during the process of DNA replication. Recently, some reports have been presented on high 8-OHdG levels in patients suffering from various diseases such as chronic hepatitis, Fanconi s anemia, diabetes mellitus and Helicobacter pylori infections. As a result, 8-OHdG is a useful marker for the study of DNA damage arising from reactive oxygen species and is of great significance for cancer research. The 8-OHdG levels in DNA can help understand the mechanism of carcinogens and lead to more effective treatments for many different types of cancer. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to 8-OHdG. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for 8-OHdG. and Avidin conjugated to 3 Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3'5, 5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain 8-OHdG., biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of 8-OHdG. in the samples is then determined by comparing the O.D. of the samples to the standard curve.

96 Strip Wells

895 USD