ELISA/assay

Human Pyruvate Kinase, M2-PK ELISA Kit

MBS700093

24-Strip-Wells (LIMIT 1)

240 USD

ELISA Kit

Human Pyruvate Kinase, M2-PK ELISA Kit

[pyruvate kinase, muscle]

[Human Pyruvate Kinase; M2-PK ELISA Kit; CTHBP; MGC3932; OIP3; PK3; PKM; TCB; THBP1; OPA-interacting protein 3; PK; muscle type; thyroid hormone-binding protein; cytosolic; pyruvate kinase; muscle]

[pyruvate kinase PKM isoform c; Pyruvate kinase PKM; pyruvate kinase PKM; p58; OIP-3; tumor M2-PK; PK, muscle type; pyruvate kinase 2/3; OPA-interacting protein 3; pyruvate kinase isozymes M1/M2; pyruvate kinase muscle isozyme; thyroid hormone-binding protein 1; epididymis secretory protein Li 30; cytosolic thyroid hormone-binding protein; thyroid hormone-binding protein, cytosolic; pyruvate kinase, muscle; Cytosolic thyroid hormone-binding protein; CTHBP; Opa-interacting protein 3; OIP-3; Pyruvate kinase 2/3; Pyruvate kinase muscle isozyme; Thyroid hormone-binding protein 1; THBP1; Tumor M2-PK; p58]

[PKM2]

[PKM; PKM; PK3; TCB; OIP3; PKM2; CTHBP; THBP1; HEL-S-30; OIP3; PK2; PK3; PKM2; CTHBP; OIP-3; THBP1]

KPYM_HUMAN

Human

605

This assay has high sensitivity and excellent specificity for detection of human LXA4. No significant cross-reactivity or interference between human LXA4 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Cell Culture Supernates, Tissue Homogenates, Cell Lysates, Urine. Assay Type: Quantitative Sandwich. Detection Range: 0.625 pg/ml-40 pg/ml. Sensitivity: 0.156 pg/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LXA4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LXA4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LXA4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LXA4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

332164775

NP_001193725.1

NM_001206796.1

P14618

57,937 Da

Adenine Ribonucleotide Biosynthesis, IMP = ADP,ATP Pathway (618580); Adenine Ribonucleotide Biosynthesis, IMP = ADP,ATP Pathway (468242); Biosynthesis Of Amino Acids Pathway (790012); Biosynthesis Of Amino Acids Pathway (795174); Carbon Metabolism Pathway (814926); Carbon Metabolism Pathway (817567); Disease Pathway (530764); Glucose Metabolism Pathway (106199); Glycogen Storage Diseases Pathway (980468); Glycolysis Pathway (105911)

This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported. [provided by RefSeq, May 2011]

Function: Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.3 PublicationsManual assertion based on experiment in:Ref.20. Catalytic activity: ATP + pyruvate = ADP + phosphoenolpyruvate.2 PublicationsManual assertion based on experiment in:Ref.11. Cofactor: Magnesium. Enzyme regulation: Isoform M2 is allosterically activated by D-fructose 1,6-bisphosphate (FBP). Inhibited by oxalate and 3,3',5-triiodo-L-thyronine (T3). The activity of the tetrameric form is inhibited by PML. Selective binding to tyrosine-phosphorylated peptides releases the allosteric activator FBP, leading to inhibition of PKM enzymatic activity, this diverts glucose metabolites from energy production to anabolic processes when cells are stimulated by certain growth factors. Glycolytic flux are highly dependent on de novo biosynthesis of serine and glycine, and serine is a natural ligand and allosteric activator of isoform M2.6 PublicationsManual assertion based on experiment in:Ref.2

24-Strip-Wells (LIMIT 1)

240 USD

48-Strip-Wells

550

96-Strip-Wells

795

5x96-Strip-Wells

2890

10x96-Strip-Wells

5465