catalog number :
MBS700091
products type :
ELISA Kit
products full name :
Human transforming growth factor alpha, TGF-alpha ELISA Kit
products short name :
[transforming growth factor, alpha]
products name syn :
[Human transforming growth factor alpha; TGF-alpha ELISA Kit; TFGA; transforming growth factor-alpha; transforming growth factor; alpha]
other names :
[protransforming growth factor alpha isoform 2 preproprotein; Protransforming growth factor alpha; protransforming growth factor alpha; TGF-alpha; transforming growth factor, alpha; EGF-like TGF; ETGF; TGF type 1]
products gene name :
[TGFA]
other gene names :
[TGFA; TGFA; TFGA; TGF-alpha; ETGF]
uniprot entry name :
TGFA_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of fish TGFB1. No significant cross-reactivity or interference between fish TGFB1 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma. Assay Type: Quantitative Sandwich. Detection Range: 6.25 ng/ml-400 ng/ml. Sensitivity: 3.12ng/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TGFB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TGFB1 present is bound by the immobilized antibody. After removing any unbound substances, a Horseradish Peroxidase (HRP)-conjugated antibody specific for TGFB1 is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TGFB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001093161.1
ncbi gb acc num :
NM_001099691.2
ncbi mol weight :
17,006 Da
ncbi pathways :
Direct P53 Effectors Pathway (137939); ErbB Receptor Signaling Network Pathway (138016); ErbB Signaling Pathway (198844); ErbB Signaling Pathway (83049); ErbB Signaling Pathway (458); FOXM1 Transcription Factor Network Pathway (137935); Glioma Pathway (83110); Glioma Pathway (522); Non-small Cell Lung Cancer Pathway (83119); Non-small Cell Lung Cancer Pathway (531)
ncbi summary :
This gene encodes a growth factor that is a ligand for the epidermal growth factor receptor, which activates a signaling pathway for cell proliferation, differentiation and development. This protein may act as either a transmembrane-bound ligand or a soluble ligand. This gene has been associated with many types of cancers, and it may also be involved in some cases of cleft lip/palate. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
uniprot summary :
Function: TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.
size1 :
24-Strip-Wells (LIMIT 1)
size5 :
10x96-Strip-Wells