Rat Atrial Natriuretic Peptide, ANP ELISA Kit | MyBioSource MBS700086 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat Atrial Natriuretic Peptide, ANP ELISA Kit

catalog :

MBS700086

quantity :

96 Strip Wells

price :

850 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS700086

products type :

ELISA Kit

products full name :

Rat Atrial Natriuretic Peptide, ANP ELISA Kit

products short name :

natriuretic peptide precursor A

products name syn :

Rat Atrial Natriuretic Peptide; ANP ELISA Kit; ANF; ANP; ATFB6; CDD-ANF; PND; atrial natriuretic factor; atriopeptin; cardionatrin; prepronatriodilatin; natriuretic peptide precursor A

other names :

natriuretic peptides A; Natriuretic peptides A; natriuretic peptides A; ANF; ANP; prepronatriodilatin; atrial natriuretic factor; atrial natriuretic peptide; natriuretic peptide precursor A; PrepronatriodilatinCleaved into the following chain:Atrial natriuretic factor; ANFAlternative name(s):Atrial natriuretic peptide; ANP

products gene name :

NPPA

other gene names :

NPPA; NPPA; ANF; ANP

uniprot entry name :

ANF_RABIT

reactivity :

Rat

sequence length :

153

specificity :

This assay has high sensitivity and excellent specificity for detection of Rat NPPA. No significant cross-reactivity or interference between Rat NPPA and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, cell culture supernates, tissue homogenates and cell lysates. Detection Range: 7.8 pg/ml-500 pg/ml. Detection Wavelength: 450 nm. Sensitivity: 1.95 pg/ml

other info2 :

Sample Volume: 50-100ul

products description :

Introduction: Atrial natriuretic peptide (ANP), atrial natriuretic factor (ANF), or atriopeptin, is a polypeptide hormone involved in the homeostatic control of body water, sodium, and adiposity. It is released by atrial myocytes, muscle cells in the atria of the heart, in response to high blood pressure. ANP acts to reduce the water, sodium and adipose loads on the circulatory system, thereby reducing blood pressure. ANP is a 28 amino acid peptide with a 17 amino acid ring in the middle of the molecule. The ring is formed by a disulfide bond between two cysteine residues at positions 7 and 23. ANP is closely related to BNP (brain natriuretic peptide) and CNP (C-type natriuretic peptide) which all share the same amino acid ring. ANP was discovered in 1981 by a team in Ottawa led by Adolfo J. de Bold after they made the seminal observation that injection of atrial (but not ventricular) tissue extracts into rats caused copious natriuresis. ANP is produced, stored and released by atrial myocytes, 3 muscle cells in the atria of the heart. It is released in response to a variety of signals induced by hypervolaemia, exercise or caloric restriction. The hormone is constitutively expressed in the ventricle in response to stress induced by increased afterload (eg. increased ventricular pressure from aortic stenosis) or injury (eg. myocardial infarction). Neutral endopeptidase (NEP)is the enzyme that metabolizes natriuretic peptides. Several inhibitors of NEP are currently being developed to treat disorders ranging from hypertension to heart failure. Most of them are dual inhibitors. Omapatrilat (dual inhibitor of NEP and Angiotensin Converting Enzyme) developed by BMS did not receive FDA approval due to angioedema safety concerns. Other dual inhibitors of NEP with ACE / angiotensin receptor are currently being developed by pharmaceutical companies. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ANP. Standards or samples are then 4 added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for ANP and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain ANP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of ANP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

126723777

ncbi acc num :

NP_001075731.1

ncbi gb acc num :

NM_001082262.1

uniprot acc num :

P07500

ncbi mol weight :

16,843 Da

ncbi pathways :

Focal Adhesion Pathway 83067!!Focal Adhesion Pathway 478!!MAPK Signaling Pathway 83048!!MAPK Signaling Pathway 456!!Salmonella Infection Pathway 375172!!Salmonella Infection Pathway 375149

uniprot summary :

Function: Hormone playing a key role in cardiovascular homeostasis through regulation of natriuresis, diuresis, and vasodilation. Also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3

size :

96 Strip Wells

price :

850 USD

more info or order :

MyBioSource product webpage