ELISA/assay

Human Neuronal Nitric Oxide Synthase, nNOS ELISA Kit

MBS700071

96 Strip Wells

850 USD

ELISA Kit

Human Neuronal Nitric Oxide Synthase, nNOS ELISA Kit

nitric oxide synthase 1 (neuronal)

Human Neuronal Nitric Oxide Synthase (nNOS) ELISA Kit; IHPS1; NOS; nNOS; nitric oxide synthase 1; neuronal; nitric oxide synthase 1 (neuronal)

nitric oxide synthase, brain isoform 1; Nitric oxide synthase, brain; nitric oxide synthase, brain; NOS type I; neuronal NOS; constitutive NOS; peptidyl-cysteine S-nitrosylase NOS1; nitric oxide synthase 1 (neuronal); Constitutive NOS; NC-NOS; NOS type I; Neuronal NOS; N-NOS; nNOS; Peptidyl-cysteine S-nitrosylase NOS1; bNOS

NOS1

NOS1; NOS1; NOS; bNOS; nNOS; IHPS1; N-NOS; NC-NOS; N-NOS; nNOS

NOS1_HUMAN

Human

1434

This assay has high sensitivity and excellent specificity for detection of Human NOS1. No significant cross-reactivity or interference between Human NOS1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma and cell culture supernates. Detection Range: 0.156 IU/ml-10 IU/ml. Detection Wavelength: 450 nm. Sensitivity: 0.039 IU/ml

Sample Volume: 50-100ul

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to nNOS. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for nNOS and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain nNOS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of nNOS in the samples is then determined by comparing the O.D. of the samples to the standard curve.

10835173

NP_000611.1

NM_000620.4

P29475

160,970 Da

Alzheimer's Disease Pathway 83097!!Alzheimer's Disease Pathway 509!!Alzheimers Disease Pathway 672448!!Amyotrophic Lateral Sclerosis (ALS) Pathway 920975!!Amyotrophic Lateral Sclerosis (ALS) Pathway 83099!!Amyotrophic Lateral Sclerosis (ALS) Pathway 511!!Arginine And Proline Metabolism Pathway 82957!!Arginine And Proline Metabolism Pathway 323!!Calcium Signaling Pathway 83050!!Calcium Signaling Pathway 459

The protein encoded by this gene belongs to the family of nitric oxide synthases, which synthesize nitric oxide from L-arginine. Nitric oxide is a reactive free radical, which acts as a biologic mediator in several processes, including neurotransmission, and antimicrobial and antitumoral activities. In the brain and peripheral nervous system, nitric oxide displays many properties of a neurotransmitter, and has been implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. This protein is ubiquitously expressed, with high level of expression in skeletal muscle. Multiple transcript variants that differ in the 5' UTR have been described for this gene but the full-length nature of these transcripts is not known. Additionally, alternatively spliced transcript variants encoding different isoforms (some testis-specific) have been found for this gene.[provided by RefSeq, Feb 2011]

Function: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR. Catalytic activity: 2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O. Cofactor: Heme group. Enzyme regulation: Stimulated by calcium/calmodulin. Inhibited by n-Nos-inhibiting protein (PIN) which may prevent the dimerization of the protein. Inhibited by NOSIP.

96 Strip Wells

850 USD