ELISA/assay

Mouse B-cell leukemia/lymphoma 2, Bcl-2 ELISA Kit

MBS700048

96 Strip Wells

895 USD

ELISA Kit

Mouse B-cell leukemia/lymphoma 2, Bcl-2 ELISA Kit

B-cell CLL/lymphoma 2

Mouse B-cell leukemia/lymphoma 2; Bcl-2 ELISA Kit; Bcl-2; B-cell lymphoma protein 2; OTTHUMP00000163680; B-cell CLL/lymphoma 2

apoptosis regulator Bcl-2 isoform 1; Apoptosis regulator Bcl-2; apoptosis regulator Bcl-2; B-cell leukemia/lymphoma 2; B cell leukemia/lymphoma 2

BCL2

Bcl2; Bcl2; Bcl-2; AW986256; C430015F12Rik; D630044D05Rik; D830018M01Rik; Bcl-2

BCL2_MOUSE

Mouse

236

This assay has high sensitivity and excellent specificity for detection of Mouse BCL2. No significant cross-reactivity or interference between Mouse BCL2 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma. Detection Range: 15.6 pg/ml-1000 pg/ml. Detection Wavelength: 450 nm. Sensitivity: 3.9 pg/ml

Sample Volume: 50-100ul. Protein Biological Process 1: Apoptosis/Autophagy. Protein Biological Process 3: Apoptosis

Introduction: Bcl-2 is the prototype for a family of mammalian genes and the proteins they produce. They govern mitochondrial outer membrane permeabilization (MOMP) and can be either pro-apoptotic (Bax, BAD, Bak and Bok among others) or anti-apoptotic (including Bcl-2 proper, Bcl-xL, and Bcl-w, among an assortment of others). There are a total of 25 genes in the Bcl-2 family known to date. The members of the Bcl-2 family share one or more of the four characteristic domains of homology entitled the Bcl-2 homology (BH) domains (named BH1, BH2, BH3 and BH4). The BH domains are known to be crucial for function, as deletion of these domains via molecular cloning affects survival/apoptosis rates. The anti-apoptotic Bcl-2 proteins, such as Bcl-2 and Bcl-xL, conserve all four BH domains. The BH domains also serve to subdivide the pro-apoptotic Bcl-2 proteins into those with several BH domains (e.g. Bax and Bak) or those proteins that have only the BH3 domain (e.g. Bid, Bim and Bad). The Bcl-2 family has a general structure that consists of a hydrophobic helix surrounded by amphipathic helices. Many members of the family have transmembrane domains. The site of action for the Bcl-2 family is mostly on the outer mitochondrial membrane. Within the mitochondria are apoptogenic factors that if released activate the executioners of apoptosis, the caspases. Depending on their function, once activated, Bcl-2 proteins either promote the release of these factors, or keep them sequestered in the mitochondria. Whereas the activated pro-apoptotic Bak and/or Bax would form MAC and mediate the release of cytochrome c, the anti-apoptotic Bcl-2 would block it, possibly through inhibition of Bax and/or Bak. There are a number of theories concerning how the Bcl-2 gene family exert their pro- or anti-apoptotic effect. An important one states that this is achieved by activation or inactivation of an inner mitochondrial permeability transition pore, which is involved in the regulation of matrix Ca2+, pH, and voltage. It is also thought that some Bcl-2 family proteins can induce or inhibit the release of cytochrome c in to the cytosol which, once there, activates caspase-9 and caspase-3, leading to apoptosis. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bcl-2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bcl-2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain Bcl-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Bcl-2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

133892547

NP_033871.2

NM_009741.4

P10417

26,407 Da

Activation Of BAD And Translocation To Mitochondria Pathway 1000434!!Activation Of BH3-only Proteins Pathway 1000433!!Adrenergic Signaling In Cardiomyocytes Pathway 908268!!Adrenergic Signaling In Cardiomyocytes Pathway 909696!!Amyotrophic Lateral Sclerosis (ALS) Pathway 83296!!Amyotrophic Lateral Sclerosis (ALS) Pathway 511!!Apoptosis Pathway 198339!!Apoptosis Pathway 83257!!Apoptosis Pathway 470!!Apoptosis Pathway 970512

Function: Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).

96 Strip Wells

895 USD