Rabbit anti-human Nephrin polyclonal Antibody | MyBioSource MBS7000356 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit anti-human Nephrin polyclonal Antibody

catalog :

MBS7000356

quantity :

0.05 mg

price :

160 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

IHC image of MBS7000356 diluted at 1:250 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

image 2 :

Western Blot. Positive WB detected in: Jurkat whole cell lysate. All lanes: NPHS1 antibody at 5.6ug/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 135, 131 kDa. Observed band size: 135 kDa

image 3 :

Immunofluorescence staining of HepG2 cells with MBS7000356 at 1:125, counter- stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

product information

catalog number :

MBS7000356

products type :

Antibody

products full name :

Rabbit anti-human Nephrin polyclonal Antibody

products short name :

[Nephrin]

products name syn :

[Nephrin (Renal glomerulus-specific cell adhesion receptor), NPHS1, NPHN]

other names :

[nephrin; Nephrin; nephrin; NPHS1 nephrin; Renal glomerulus-specific cell adhesion receptor]

products gene name syn :

[NPHS1]

other gene names :

[NPHS1; NPHS1; CNF; NPHN; nephrin; NPHN]

uniprot entry name :

NPHN_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human

purity :

>95%, Protein G purified

form :

Liquid

storage stability :

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

tested application :

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

app notes :

Recommended dilution:. WB:1:500-1:5000. IHC:1:200-1:500. IF:1:50-1:200

image1 heading :

Immunohistochemistry (IHC)

image2 heading :

Western Blot (WB)

image3 heading :

Immunofluorescence (IF)

other info1 :

Immunogen: Recombinant Human Nephrin protein (23-257AA). Conjugate: Non-conjugated

other info2 :

Buffer: Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4. Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-19000 / sc-32529 / sc-32530 / sc-32532 / sc-28192. Species: Human. Research Area: Signal Transduction. Target Names: NPHS1

products description :

Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion.

ncbi gi num :

4758822

ncbi acc num :

NP_004637.1

ncbi gb acc num :

NM_004646.3

uniprot acc num :

O60500

ncbi pathways :

Cell-Cell Communication Pathway (1270231); Nephrin Interactions Pathway (1270243); Nephrin/Neph1 Signaling In The Kidney Podocyte Pathway (138072)

ncbi summary :

This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. Mutations in this gene result in Finnish-type congenital nephrosis 1, characterized by severe proteinuria and loss of the slit diaphragm and foot processes.[provided by RefSeq, Oct 2009]

uniprot summary :

NPHS1: Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion. Interacts with CD2AP (via C-terminal domain). Interacts with MAGI1 (via PDZ 2 and 3 domains) forming a tripartite complex with IGSF5/JAM4. Interacts with DDN; the interaction is direct. Self-associates (via the Ig-like domains). Also interacts (via the Ig-like domains) with KIRREL/NEPH1 and KIRREL2; the interaction with KIRREL is dependent on KIRREL glycosylation. Forms a complex with ACTN4, CASK, IQGAP1, MAGI2, SPTAN1 and SPTBN1. Interacts with NPHS2. Specifically expressed in podocytes of kidney glomeruli. Belongs to the immunoglobulin superfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Immunoglobulin superfamily; Cell adhesion. Chromosomal Location of Human Ortholog: 19q13.1. Cellular Component: cell projection; integral to plasma membrane; intracellular; plasma membrane. Molecular Function: myosin binding; protein binding. Biological Process: cell adhesion; excretion; glomerular basement membrane development; JNK cascade; myoblast fusion; positive regulation of actin filament polymerization; skeletal muscle development. Disease: Nephrotic Syndrome, Type 1

size1 :

0.05 mg

price1 :

160 USD

size2 :

0.1 mg

price2 :

235

more info or order :

MyBioSource product webpage