catalog number :
MBS700030
products type :
ELISA Kit
products full name :
Human Complement 4, C4 ELISA Kit
products short name :
Complement 4, C4
products name syn :
Human Complement 4; C4 ELISA Kit; Complement 4; C4
other names :
complement 4; complement 4; complement C4; MHC-linked complement C4; complement 4
other gene names :
C4; C4-2
specificity :
This assay has high sensitivity and excellent specificity for detection of human C4. No significant cross-reactivity or interference between human C4 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.41 ng/ml -300 ng/ml. Sensitivity: The minimum detectable dose of human C4 is typically less than 0.41 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest human C4 concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
. Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for C4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any C4 present is bound by the immobilized antibody. After removing any unbound substances, a Horseradish Peroxidase (HRP)-conjugated antibody specific for C4 is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of C4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001070701.1
ncbi gb acc num :
NM_001077233.1
size4 :
10x96-Strip-Wells