ELISA/assay

Human Corticosteroid Binding Globulin, CBG ELISA Kit

MBS700017

48-Strip-Wells

510 USD

ELISA Kit

Human Corticosteroid Binding Globulin, CBG ELISA Kit

serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin) , member 6

Human Corticosteroid Binding Globulin (CBG) ELISA Kit; CBG; corticosteroid binding globulin; serine (or cysteine) proteinase inhibitor; clade A (alpha-1 antiproteinase; antitrypsin); member 6; transcortin; serpin peptidase inhibitor; clade A (alpha-1 antiproteinase; antitrypsin); member 6

corticosteroid-binding globulin; Corticosteroid-binding globulin; corticosteroid-binding globulin; serpin A6; transcortin; corticosteroid binding globulin; serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 6; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 6; Serpin A6; Transcortin

SERPINA6

SERPINA6; SERPINA6; CBG; CBG; CBG

CBG_HUMAN

Human

405

This assay has high sensitivity and excellent specificity for detection of human CBG. No significant cross-reactivity or interference between human CBG and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Competitive. Detection Range: 20 pmol/ml-8000 pmol/ml. Sensitivity: The minimum detectable dose of human CBG is typically less than 20 pmol/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest human CBG concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for CBG and Horseradish Peroxidase (HRP) conjugated CBG. The competitive inhibition reaction is launched between with HRP labeled CBG and unlabeled CBG with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of CBG in the sample. The color development is stopped and the intensity of the color is measured.

73858564

NP_001747.2

NM_001756.3

P08185

45,141 Da

Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)

This gene encodes an alpha-globulin protein with corticosteroid-binding properties. This is the major transport protein for glucorticoids and progestins in the blood of most vertebrates. The gene localizes to a chromosomal region containing several closely related serine protease inhibitors which may have evolved by duplication events. [provided by RefSeq, Jul 2008]

Function: Major transport protein for glucocorticoids and progestins in the blood of almost all vertebrate species.1 PublicationManual assertion based on experiment in:Ref.10

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800