catalog number :
MBS700013
products type :
ELISA Kit
products full name :
Rat Interleukin 11, IL-11 ELISA Kit
products short name :
interleukin 11
products name syn :
Rat Interleukin 11; IL-11 ELISA KIT; AGIF; IL-11; adipogenesis inhibitory factor; oprelvekin; interleukin 11
other names :
Interleukin-11; Interleukin-11; interleukin-11; interleukin 11
products gene name :
IL11
other gene names :
Il11; Il11; Il-11; IL-11
uniprot entry name :
IL11_RAT
specificity :
This assay has high sensitivity and excellent specificity for detection of rat IL-11. No significant cross-reactivity or interference between rat IL-11 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml -1000 pg/ml. Sensitivity: The minimum detectable dose of rat IL-11 is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL-11 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-11 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-11 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-11 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi mol weight :
21,555 Da
ncbi pathways :
Cytokine-cytokine Receptor Interaction Pathway (83443); Cytokine-cytokine Receptor Interaction Pathway (460); Cytokines And Inflammatory Response (BioCarta) Pathway (198521); Hematopoietic Cell Lineage Pathway (83470); Hematopoietic Cell Lineage Pathway (489); Jak-STAT Signaling Pathway (83469); Jak-STAT Signaling Pathway (488); Rheumatoid Arthritis Pathway (200306); Rheumatoid Arthritis Pathway (200269)
ncbi summary :
enhances cellular recovery after ischemia-reperfusion injury [RGD, Feb 2006]
uniprot summary :
Function: Directly stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production
size4 :
10x96-Strip-Wells