catalog number :
MBS700010
products type :
ELISA Kit
products full name :
Human Netrin-4, Ntn4 ELISA Kit
products short name :
netrin 4
products name syn :
Human Netrin-4; Ntn4 ELISA Kit; FLJ23180; PRO3091; Netrin-4; beta-netrin; netrin 4
other names :
netrin-4; Netrin-4; netrin-4; beta-netrin; hepar-derived netrin-like protein; netrin 4; Beta-netrin; Hepar-derived netrin-like protein
products gene name :
NTN4
other gene names :
NTN4; NTN4; PRO3091
uniprot entry name :
NET4_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of Human NTN4. No significant cross-reactivity or interference between Human NTN4 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, cell culture supernates, tissue homogenates. Detection Range: 0.312 ng/ml-20 ng/ml. Detection Wavelength: 450 nm. Sensitivity: 0.078 ng/ml
other info2 :
Sample Volume: 50-100ul
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for netrin-4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any netrin-4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for netrin-4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of netrin-4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_067052.2
ncbi gb acc num :
NM_021229.3
ncbi mol weight :
70,071 Da
ncbi pathways :
Axon Guidance Pathway 83065!!Axon Guidance Pathway 476!!Axon Guidance Pathway 105688!!Developmental Biology Pathway 477129!!Extracellular Matrix Organization Pathway 576262!!Netrin-1 Signaling Pathway 160999!!Non-integrin Membrane-ECM Interactions Pathway 833810
ncbi summary :
NTN4 belongs to a family of proteins related to laminins (see LAMA1, MIM 150320) Koch et al. (2000) [PubMed 11038171].[supplied by OMIM, Mar 2008]
uniprot summary :
Function: May play an important role in neural, kidney and vascular development. Promotes neurite elongation from olfactory bulb explants.1 PublicationManual assertion based on experiment in:Ref.1