catalog number :
MBS700009
products type :
ELISA Kit
products full name :
Pig Interferon alpha, IFN-alpha ELISA Kit
products short name :
Interferon alpha, IFN-alpha
products name syn :
Pig Interferon alpha; IFN-alpha ELISA Kit; Interferon alpha; IFN-alpha
other names :
interferon-alpha; Interferon omega-1; interferon omega-1; interferon alpha-II-1; IFN-omega 1, interferon omega-1; interferon, omega 1; Interferon alpha-II-1
products gene name :
IFN-alpha
other gene names :
IFNW1; IFNW1
uniprot entry name :
IFNW1_HUMAN
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Sandwich. Detection Range: 7.8 pg/ml-500 pg/ml. Sensitivity: 1.95 pg/ml
other info2 :
Detection Wavelength: 450 nm. Sample Volume: 50-100ul
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IFN-alpha has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IFN-alpha present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IFN-alpha is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IFN-alpha bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
AAA52724.1
ncbi mol weight :
22,319 Da
ncbi pathways :
Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Jak-STAT Signaling Pathway (83077); Jak-STAT Signaling Pathway (488); RIG-I-like Receptor Signaling Pathway (117292); RIG-I-like Receptor Signaling Pathway (116128)
uniprot summary :
IFNW1: Belongs to the alpha/beta interferon family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 9p22. Cellular Component: extracellular space. Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor binding; interferon-alpha/beta receptor binding; cytokine activity. Biological Process: B cell proliferation; adaptive immune response; cytokine and chemokine mediated signaling pathway; response to virus; regulation of MHC class I biosynthetic process; humoral immune response; T cell activation during immune response; response to exogenous dsRNA; B cell differentiation; natural killer cell activation during immune response; innate immune response; cell cycle arrest; defense response to virus; positive regulation of peptidyl-serine phosphorylation of STAT protein
size4 :
10x96-Strip-Wells