protein

Human KLF4-TAT

MBS696201

0.005 mg

125 USD

Recombinant Protein

Human KLF4-TAT

[KLF4-TAT]

[Kruppel-like factore-4, EZF, GKLF]

[Krueppel-like factor 4; Epithelial zinc finger protein EZF; Gut-enriched krueppel-like factor]

[KLF4-TAT]

[KLF4; EZF; GKLF]

KLF4_HUMAN

E.coli

Species Reactivity: Human

MAVSDALLP SFSTFASGP AGREKTLRQ AGAPNNRWR EELSHMKRL PPVLPGRPY DLAAATVAT DLESGGAGA ACGGSNLAP LPRRETEEF NDLLDLDFI LSNSLTHPP ESVAATVSS SASASSSSS PSSSGPASA PSTCSFTYP IRAGNDPGV APGGTGGGL LYGRESAPP PTAPFNLAD INDVSPSGG FVAELLRPE LDPVYIPPQ QPQPPGGGL MGKFVLKAS LSAPGSEYG SPSVISVSK GSPDGSHPV VVAPYNGGP PRTCPKIKQ EAVSSCTHL GAGPPLSNG HRPAAHDFP LGRQLPSRT TPTLGLEEV LSSRDCHPA LPLPPGFHP HPGPNYPSF LPDQMQPQV PPLHYQELM PPGSCMPEE PKPKRGRRS WPRKRTATH TCDYAGCGK TYTKSSHLK AHLRTHTGE KPYHCDWDG CGWKFARSD ELTRHYRKH TGHRPFQCQ KCDRAFSRS DHLALHMKR HFGGYGRKK RRQRRR

> or = 90% as determined by SDS-PAGE gel and HPLC Analysis.

Sterile filtered through a 0.2 micron filter. Lyophilized from 10mM Sodium Phosphate, pH 8.0.

At -20 degree C

Authenticity: Verified by N-terminal and Mass Spectrometry analyses (when applicable). Endotoxin: Endotoxin level is <0.1 ng/ug of protein <1 EU/ug). Protein: Verified by UV Spectroscopy and/or SDS-PAGE gel. Biological Activity: Testing in progress.

Recomstitution: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1 % BSA) and store in working aliquots at -20°C to -80°C.

Proliferation and Cell Cycle

KLF4 is a member of the Kruppel-like factor (KLF) family of zinc finger transcription factors. Members of this family have in common 3 contiguous C2H2-type zinc fingers at the carboxyl terminus that comprise the DNA-binding domain. KLF4 is highly expressed in skin and gut epithelial tissues, but is also found in various other cells and tissues, including vascular endothelial cells, lymphocytes, lung, and testis. It is an important regulator of the cell cycle, transcription, and cell differentiation. Together with Sox2, Oct4, and cMyc, KLF4 can induce the reprogramming of primary human fibroblasts to a pluripotent state. KLF4 and other transcription factors can be introduced into cells by DNA transfection, viral infection, or microinjection. Protein transduction using TAT fusion proteins represents an alternative methodology for introducing transcription factors into primary as well as transformed cells. Recombinant human KLF4-TAT is a 51.7 kDa protein containing 483 amino acid residues, including 13- residue C-terminal TAT peptide.

O43474

6,489 Da

KLF4: Transcription factor; can act both as activator and as repressor. Binds the 5 -CACCC-3 core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription. Interacts with POU5F1/OCT4 and SOX2. Interacts with MUC1 (via the C-terminal domain). Belongs to the krueppel C2H2-type zinc-finger protein family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Transcription factor; DNA-binding; C2H2-type zinc finger protein. Chromosomal Location of Human Ortholog: 9q31. Cellular Component: nucleoplasm; transcription factor complex; nuclear chromatin. Molecular Function: protein binding; zinc ion binding; double-stranded DNA binding; phosphoinositide 3-kinase regulator activity; transcription factor activity. Biological Process: transcription from RNA polymerase II promoter; fat cell differentiation; post-embryonic camera-type eye development; positive regulation of nitric oxide biosynthetic process; somatic stem cell maintenance; negative regulation of smooth muscle cell proliferation; positive regulation of transcription, DNA-dependent; positive regulation of cellular protein metabolic process; negative regulation of interleukin-8 biosynthetic process; negative regulation of muscle hyperplasia; negative regulation of transcription from RNA polymerase II promoter; negative regulation of caspase activity; positive regulation of protein metabolic process; regulation of phosphoinositide 3-kinase activity; positive regulation of hemoglobin biosynthetic process; negative regulation of cell proliferation; post-embryonic hemopoiesis; regulation of cell differentiation; epidermis morphogenesis; response to retinoic acid; negative regulation of protein kinase B signaling cascade; negative regulation of heterotypic cell-cell adhesion; stem cell maintenance; positive regulation of telomerase activity; inhibition of NF-kappaB transcription factor; negative regulation of inflammatory response; epidermal cell differentiation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; mesodermal cell fate determination; negative regulation of phosphoinositide 3-kinase cascade

0.005 mg

125 USD

0.025 mg

195

0.1 mg

505

0.5 mg

1410

1 mg

2320