Recombinant Human MMP-1 | MyBioSource MBS696181 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Human MMP-1

catalog :

MBS696181

quantity :

0.002 mg

price :

125 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS696181

products type :

Recombinant Protein

products full name :

Recombinant Human MMP-1

products short name :

[MMP-1]

products name syn :

[Matrix metalloproteinase-1; Fibroblast collagenase; interstitial collagenase]

other names :

[Interstitial collagenase; Interstitial collagenase; interstitial collagenase; fibroblast collagenase; matrix metalloprotease 1; matrix metalloproteinase 1; matrix metallopeptidase 1 (interstitial collagenase); Fibroblast collagenase; Matrix metalloproteinase-1; MMP-1]

products gene name :

[MMP-1]

other gene names :

[MMP1; MMP1; CLG; CLGN; CLG; MMP-1]

uniprot entry name :

MMP1_HUMAN

reactivity :

Species Reactivity: Human

sequence :

MFVLTEGNP RWEQTHLTY RIENYTPDL PRADVDHAI EKAFQLWSN VTPLTFTKV SEGQADIMI SFVRGDHRD NSPFDGPGG NLAHAFQPG PGIGGDAHF DEDERWTNN FREYNLHRV AAHELGHSL GLSHSTDIG ALMYPSYTF SGDVQLAQD DIDGIQAIY GRSQNPVQP IGPQTPKAC DSKLTFDAI TTIRGEVMF FKDRFYMRT NPFYPEVEL NFISVFWPQ LPNGLEAAY EFADRDEVR FFKGNKYWA VQGQNVLHG YPKDIYSSF GFPRTVKHI DAALSEENT GKTYFFVAN KYWRYDEYK RSMDPGYPK MIAHDFPGI GHKVDAVFM KDGFFYFFH GTRQYKFDP KTKRILTLQ KANSWFNCR KN

purity :

>= 98% by SDS-PAGE gel and HPLC analyses

form :

Sterile filtered through a 0.2 micron filter. Lyophilized from 10mM Sodium Phosphate, pH 7.5 + 0.1mm Calcium Chloride+ 0.2mM EDTA + 0.1% CHAPS

storage stability :

Store at -20 degree C

other info1 :

Source: E.coli. Biological Activity: MMP-1 activity was measured by its ability to cleave a chromogenic peptide MMP-1 substrate at room temperature. At an MMP-1 concentration of 2.5 mug/ml, 50% cleavage was achieved at an incubation time of approximately 25 minutes. Authenticity: Verified by N-terminal and Mass Spectromtry analyses (when applicable)

other info2 :

Endotoxin: Endotoxin levels is <0.1 ng/ug of protein (<1EU/ug). Protein Content: Verified by UV spectroscopy and/or SDS-PAGE gel. Reconstitution: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.5-1.0 mg/ml. Slow to dissolve. Gently shake the solution for about 10 minutes for better solubility. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20 degree C to -80 degree C. Avoid repeat freeze that cycles. Country of Origin: USA

products categories :

Cancer; Angiogenesis; Bone Biology

products description :

Matrix metalloproteinases (MMPs) are a family of endoproteases that require zinc and calcium for expressing catalytic activity. These enzymes play a central role in the maintenance and remodeling of the extracellular matrix. Elevated expression of their activity, caused either by up-regulation of their expression or down-regulation of their cognate inhibitors, has been implicated in various degenerative disorders, including arthritis, cardiovascular disease, skeletal growth-plate disorders, and cancer metastasis. MMP-1 is a secreted collagenase with specificity toward Type I, II, III, VII, and X collagens. Recombinant human MMP-1 is a 42.7 kDa protein containing the entire catalytic N-terminal domain and the C-terminal domain which is involved in substrate specificity and in binding TIMP-1.

ncbi gi num :

116852

ncbi acc num :

P03956.3

uniprot acc num :

P03956

ncbi pathways :

Activation Of Matrix Metalloproteinases Pathway (576264); Basigin Interactions Pathway (106065); Bladder Cancer Pathway (83115); Bladder Cancer Pathway (527); Cell Surface Interactions At The Vascular Wall Pathway (106062); Collagen Degradation Pathway (730309); Degradation Of The Extracellular Matrix Pathway (576263); Endothelins Pathway (137958); Extracellular Matrix Organization Pathway (576262); Glucocorticoid Receptor Regulatory Network Pathway (138014)

ncbi summary :

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes a secreted enzyme which breaks down the interstitial collagens, types I, II, and III. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Mar 2009]

uniprot summary :

MMP1: Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat s mediated neurotoxicity. Belongs to the peptidase M10A family. Protein type: Secreted, signal peptide; Protease; Secreted; Motility/polarity/chemotaxis; EC 3.4.24.7. Chromosomal Location of Human Ortholog: 11q22.3. Cellular Component: proteinaceous extracellular matrix; extracellular region. Molecular Function: zinc ion binding; metalloendopeptidase activity; endopeptidase activity; calcium ion binding. Biological Process: extracellular matrix disassembly; positive regulation of protein oligomerization; collagen catabolic process; extracellular matrix organization and biogenesis; cellular protein metabolic process; viral reproduction; proteolysis; blood coagulation; leukocyte migration. Disease: Epidermolysis Bullosa Dystrophica, Autosomal Recessive; Pulmonary Disease, Chronic Obstructive

size1 :

0.002 mg

price1 :

125 USD

size2 :

0.01 mg

price2 :

195

size3 :

0.1 mg

price3 :

840

size4 :

0.5 mg

price4 :

2410

size5 :

1 mg

price5 :

3960

more info or order :

MyBioSource product webpage