protein

Murine RANTES (CCL5)

MBS696166

0.005 mg

125 USD

Recombinant Protein

Murine RANTES (CCL5)

[RANTES]

[Recombinant Murine RANTES (CCL5), Regulation upon Activation Normal T-cells Express Sequence, CCL5, SIS-delta]

[C-C motif chemokine 5; C-C motif chemokine 5; C-C motif chemokine 5; SIS-delta; small inducible cytokine A5; small-inducible cytokine A5; T-cell-specific protein RANTES; chemokine (C-C motif) ligand 5; MuRantes; SIS-delta; Small-inducible cytokine A5; T-cell-specific protein RANTES]

[RANTES]

[CCL5]

[Ccl5; Ccl5; SISd; Scya5; RANTES; TCP228; MuRantes; Scya5]

CCL5_MOUSE

E Coli

SPYGSDTTP CCFAYLSLA LPRAHVKEY FYTSSKCSN LAVVFVTRR NRQVCANPE KKWVQEYIN YLEMS

>=98% by SDS-PAGE gel and HPLC analyses.

Sterile filtered through a 0.2 micron filter. Lyophilized with no additives.

Store at -20°C.

Testing Data

Authenticity: Verified by N-terminal and Mass Spectometry analyses (when applicable). Biological Activity: Determined by its ability to chemoattract total human lymphocyte population and total murine T cell population using a concentration range of 1.0-10.0 ng/ml. Endotoxin: Endotoxin level is <0.1 ng/ug of protein (<1EU/ug). Protein Content: Verified by UV Spectroscopy and /or SDS-PAGE gel.

Reconstitution: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL/ Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, It is recommended to furter dilute in a buffer containing a carrier protein example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Innate Immunity; Immunology; Angiogenesis; Neurosciences; Chemotaxis

RANTES is a CC-chemokine that can signal through the CCR1, CCR3, CCR5 and US28 (cytomegalovirus receptor) receptors. It is a chemoattractant towards monocytes, memory T cells (CD4+/CD45RO), basophils, and eosinophils. RANTES also has the capability to inhibit certain strains of HIV-1, HIV-2 and simian immunodeficiency virus (SIV). Recombinant murine RANTES is a 7.8 kDa protein containing 68 amino acid residues including the four highly conserved cysteine residues present in the CC chemokines.

548926

P30882.2

P30882

Chagas Disease (American Trypanosomiasis) Pathway (147810); Chagas Disease (American Trypanosomiasis) Pathway (147795); Chemokine Receptors Bind Chemokines Pathway (1000944); Chemokine Signaling Pathway (99271); Chemokine Signaling Pathway (672435); Chemokine Signaling Pathway (96864); Class A/1 (Rhodopsin-like Receptors) Pathway (1000942); Cytokine-cytokine Receptor Interaction Pathway (83248); Cytokine-cytokine Receptor Interaction Pathway (460); Cytosolic DNA-sensing Pathway (125146)

CCL5: Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. Binds to CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T- cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) and is generated by an unidentified enzyme associated with monocytes and neutrophils. By mitogens. T-cell and macrophage specific. Belongs to the intercrine beta (chemokine CC) family. Protein type: Cell adhesion; Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted; Chemokine. Cellular Component: extracellular space; cell; cytoplasm; extracellular region; intracellular. Molecular Function: heparin binding; protein homodimerization activity; protein self-association; cytokine activity; receptor signaling protein tyrosine kinase activator activity; phosphoinositide phospholipase C activity; protein kinase activity; CCR1 chemokine receptor binding; chemokine activity; chemokine receptor binding; chemokine receptor antagonist activity; chemoattractant activity; phospholipase activator activity; CCR5 chemokine receptor binding. Biological Process: exocytosis; positive regulation of cell adhesion; positive regulation of osteoclast differentiation; response to toxin; positive regulation of smooth muscle cell proliferation; positive regulation of smooth muscle cell migration; chemotaxis; positive regulation of cell-cell adhesion mediated by integrin; protein amino acid phosphorylation; positive regulation of homotypic cell-cell adhesion; pseudopodium formation; leukocyte adhesion; cell-cell signaling; positive chemotaxis; calcium ion transport; protein kinase B signaling cascade; lipopolysaccharide-mediated signaling pathway; positive regulation of T cell proliferation; inflammatory response; lymphocyte chemotaxis; protein tetramerization; phospholipase D activation; neutrophil activation; negative regulation of G-protein coupled receptor protein signaling pathway; MAPKKK cascade; positive regulation of cellular biosynthetic process; positive regulation of phosphoinositide 3-kinase cascade; cellular calcium ion homeostasis; G-protein coupled receptor protein signaling pathway; positive regulation of angiogenesis; cellular protein complex assembly; negative regulation of viral genome replication; positive regulation of tyrosine phosphorylation of STAT protein; response to cytokine stimulus; positive regulation of fever; eosinophil chemotaxis; immune response; positive regulation of neuron differentiation; positive regulation of calcium ion transport; positive regulation of defense response to virus by host; regulation of insulin secretion; positive regulation of phosphorylation; positive regulation of epithelial cell proliferation; regulation of T cell activation; positive regulation of cell migration

0.005 mg

125 USD

0.02 mg

195

0.1 mg

505

0.5 mg

1410

1 mg

2320