protein

Human Galectin-3

MBS696161

0.01 mg

125 USD

Recombinant Protein

Human Galectin-3

[Galectin-3]

[Galactose-specific lectin-3, IgE-binding protein, MAC2, L29, CPB-35]

[Galectin-3; Galectin-3; galectin-3; lectin L-29; 35 kDa lectin; MAC-2 antigen; IgE-binding protein; laminin-binding protein; galactose-specific lectin 3; carbohydrate-binding protein 35; lectin, galactoside-binding, soluble, 3; 35 kDa lectin; Carbohydrate-binding protein 35; CBP 35; Galactose-specific lectin 3; Galactoside-binding protein; GALBP; IgE-binding protein; L-31; Laminin-binding protein; Lectin L-29; Mac-2 antigen]

[LGALS3; LGALS3; L31; GAL3; MAC2; CBP35; GALBP; GALIG; LGALS2; MAC2; Gal-3; CBP 35; GALBP]

LEG3_HUMAN

E Coli

Species Reactivity: Human

ADNFSLHDA LSGSGNPNP QGWPGAWGN QPAGAGGYP GASYPGAYP GQAPPGAYP GQAPPGAYH GAPGAYPGA PAPGVYPGP PSGPGAYPS SGQPSAPGA YPATGPYGA PAGPLIVPY NLPLPGGVV PRMLITILG TVKPNANRI ALDFQRGND VAFHFNPRF NENNRRVIV CNTKLDNNW GREERQSVF PFESGKPFK IQVLVEPDH FKVAVNDAH LLQYNHRVK KLNEISKLG ISGDIDLTS ASYTMI

> =98% by SDS-PAGE gel and HPLC analyses.

Sterile filtered through a 0.2 micron filter. Lyophilized from 0.5x PBS, pH .2

At -20 degree C

Biological Activity: Determined by its ability to chemoattract human blood monocytes. Chemotactic activity was observed at a concentration of 2.5 ug/ml with a peak response obtained at 250 ug/ml. Authenticity: Verified by N-terminal and Mass Spectrometry analyses (when applicable). Endotoxin: Endotoxin level is <0.1 ng/ug of protein <1 EU/ug). Protein Content: Verified by UV Spectroscopy andlor SOS-PAGE gel.

Cell Type: Monocytes and Macrophages, T Cells. Reconstitution: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vorlex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20 degree C to -80 degree C.

Innate Immunity; Immunology; Cancer; Inflammation; Proliferation and Cell Cycle

Lectins, of either plant or animal origin, are carbohydrate binding proteins that interact with glycoprotein and glycolipids on the surface of animal cells. The Galectins are lectins that recognize and interact with beta-galactoside moieties. Galectin-3 regulates a number of biological processes, including embryogenesis, inflammatory responses, cell progression and metastasis. Galectin-3 is normally expressed in epithelia of a variety of tissues, including colon and endometrium, and in various inflammatory cells, including macrophages. Galectin-3 can function intracellularly, in controlling cell cycle and preventing T-cell apoptosis, and also extracellularly, in activating various cells, including monocytes/macrophages, mast cells, neutrophils, and lymphocytes. Expression of Galectin-3 is affected by neoplastic transformation, being up-regulated in certain types of lymphomas, and in thyroid and hepatic carcinomas. Conversely, it is down-regulated in other cancers, such as colon, breast, ovarian, and uterine. Recombinant human Galectin-3 is a globular 26.0kDa protein containing 250 amino acid residues, but no disulfide bonds.

215274262

P17931.5

P17931

AGE/RAGE Pathway (698754); Advanced Glycosylation Endproduct Receptor Signaling Pathway (187092); Hedgehog Signaling Events Mediated By Gli Proteins Pathway (137912); Immune System Pathway (106386); Innate Immune System Pathway (106387); Spinal Cord Injury Pathway (739007)

This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. The protein exhibits antimicrobial activity against bacteria and fungi. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Oct 2014]

Galectin-3: galactose-specific lectin which binds IgE. Expressed at a high level in the colonic epithelium. Also abundant in activated macrophages. Protein type: Motility/polarity/chemotaxis; Extracellular matrix; Cell surface. Chromosomal Location of Human Ortholog: 14q22.3. Cellular Component: spliceosome; extracellular matrix; extracellular space; proteinaceous extracellular matrix; membrane; mitochondrial inner membrane; cytoplasm; plasma membrane; immunological synapse; nucleus; external side of plasma membrane. Molecular Function: protein binding; laminin binding; IgE binding; carbohydrate binding; chemoattractant activity. Biological Process: neutrophil chemotaxis; extracellular matrix organization and biogenesis; RNA splicing; negative regulation of endocytosis; regulation of T cell proliferation; negative regulation of T cell receptor signaling pathway; macrophage chemotaxis; monocyte chemotaxis; epithelial cell differentiation; positive chemotaxis; eosinophil chemotaxis; innate immune response; mRNA processing; skeletal development

0.01 mg

125 USD

0.05 mg

195

0.1 mg

275

0.5 mg

730

1 mg

1180