Human FGF-basic (154 a.a.) | MyBioSource MBS696130 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Human FGF-basic (154 a.a.)

catalog :

MBS696130

quantity :

0.01 mg

price :

125 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS696130

products type :

Recombinant Protein

products full name :

Human FGF-basic (154 a.a.)

products short name :

[FGF-basic]

products name syn :

[Recombinant Human FGF-basic (154 a.a.), Fibroblast Growth Factor-basic, FGF-2, HBGF-2, Prostatropin.]

other names :

[Fibroblast growth factor 2; Fibroblast growth factor 2; fibroblast growth factor 2; prostatropin; heparin-binding growth factor 2; basic fibroblast growth factor bFGF; fibroblast growth factor 2 (basic); Basic fibroblast growth factor; bFGF; Heparin-binding growth factor 2; HBGF-2]

products gene name :

[FGF]

other gene names :

[FGF2; FGF2; BFGF; FGFB; FGF-2; HBGF-2; FGFB; FGF-2; bFGF; HBGF-2]

uniprot entry name :

FGF2_HUMAN

host :

E Coli

sequence :

AAGSITTLPA LPEDGGSGAF PPGHFKDPKR LYCKNGGFFL RIHPDGRVDG VREKSDPHIK LQLQAEERGV VSIKGVCANR YLAMKEDGRL LASKCTDEC EFFERLESNN YNTYRSRKYT SWYVALKRTG QYKLGSKTGP GQKAILFLPM SAKS

purity :

>=95% by SDS-PAGE gel and HPLC analyses.

form :

Sterile filtered through a 0.2 micron filter . Lyophilized from 5 mM Tris,pH 7.6 + 150 mM NaCI.

storage stability :

Store at -20°C

other info1 :

Authenticity: Verified by N-terminal and Mass Spectometry analyses (when applicable). Endotoxin: Endotoxin level is <0.1 ng/ug of protein (<1 EU/ug). Protein Content: Verified by UV Spectometry and/or SDS-PAGE gel.

other info2 :

Biological Activity: Assay#1: Determined by the dosedependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors. The expected ED 50 is = 2 x 10 6 units/mg. Assay#2: Determined by a cell profileration assay using Balb/c 3T3 cells. The expected ED/ 50 is = 1 x 10 7 units/mg. Reconstitution: Centrifuge the vial prior to opening. Recostitute in 5 mM Tris, pH 7.6 to a concentration of 0.1-1.0 mg/mL. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C . Avoid repeated freeze-thaw cycles.

products categories :

Stem Cell Research; Cancer; Angiogenesis; Neurosciences; Inflammation; Proliferation and Cell Cycle

products description :

FGF-basic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-basic is a non-glycosylated heparin binding growth factor that is expressed in the brain, pituitary, kidney, retina, bone, testis, adrenal gland liver, monocytes, epithelial cells and endothelial cells. FGF-basic signals through FGFR 1b, 1c, 2c, 3c and 4. Recombinant human FGF-basic is a 17.2 kDa protein consisting of 154 amino acid residues.

ncbi gi num :

261260095

ncbi acc num :

P09038.3

uniprot acc num :

P09038

ncbi pathways :

Activated Point Mutants Of FGFR2 Pathway (645281); Adaptive Immune System Pathway (366160); Angiogenesis Pathway (198772); Angiopoietin Receptor Tie2-mediated Signaling Pathway (137917); Cardiac Progenitor Differentiation Pathway (712094); Constitutive PI3K/AKT Signaling In Cancer Pathway (685535); DAP12 Interactions Pathway (685549); DAP12 Signaling Pathway (685550); Developmental Biology Pathway (477129); Disease Pathway (530764)

ncbi summary :

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. [provided by RefSeq, Jul 2008]

uniprot summary :

FGF2: Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Interacts with CSPG4, FGFBP1 and TEC. Found in a complex with FGFBP1, FGF1 and FGF2. Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non- cancerous liver tissue. Belongs to the heparin-binding growth factors family. 4 isoforms of the human protein are produced by alternative initiation. Protein type: Activator; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 4q26. Cellular Component: extracellular space; extracellular region; nucleus. Molecular Function: heparin binding; protein binding; ligand-dependent nuclear receptor transcription coactivator activity; growth factor activity; cytokine activity; chemoattractant activity; fibroblast growth factor receptor binding. Biological Process: extracellular matrix organization and biogenesis; wound healing; nerve growth factor receptor signaling pathway; somatic stem cell maintenance; activation of MAPK activity; positive regulation of transcription, DNA-dependent; chemotaxis; signal transduction; hyaluronan catabolic process; growth factor dependent regulation of satellite cell proliferation; positive regulation of MAP kinase activity; positive chemotaxis; positive regulation of cell proliferation; embryonic morphogenesis; positive regulation of cardiac muscle cell proliferation; epidermal growth factor receptor signaling pathway; nervous system development; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; positive regulation of cell fate specification; positive regulation of blood vessel endothelial cell migration; positive regulation of phosphoinositide 3-kinase activity; negative regulation of blood vessel endothelial cell migration; regulation of angiogenesis; positive regulation of angiogenesis; organ morphogenesis; cell migration during sprouting angiogenesis; ureteric bud branching; release of sequestered calcium ion into cytosol; positive regulation of cell division; Ras protein signal transduction; insulin receptor signaling pathway; phosphatidylinositol biosynthetic process; innate immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of endothelial cell proliferation; inositol phosphate biosynthetic process

size1 :

0.01 mg

price1 :

125 USD

size2 :

0.05 mg

price2 :

195

size3 :

0.1 mg

price3 :

220

size4 :

0.5 mg

price4 :

415

size5 :

1 mg

price5 :

655

more info or order :

MyBioSource product webpage