protein

Human PDGF-AA

MBS695871

0.002 mg

125 USD

Recombinant Protein

Human PDGF-AA

[PDGF-AA]

[Plateled-Derived Growth Factor-AA, Glioma-derived growth factorr (GDGF), Osteosarcoma-derived Growth Factor (ODGF)]

[Platelet-derived growth factor subunit A; Platelet-derived growth factor subunit A; platelet-derived growth factor subunit A; PDGF-1; PDGF A-chain; PDGF subunit A; platelet-derived growth factor A chain; platelet-derived growth factor alpha chain; platelet-derived growth factor alpha isoform 2 preproprotein; platelet-derived growth factor alpha polypeptide; PDGF-1; Platelet-derived growth factor A chain; Platelet-derived growth factor alpha polypeptide]

[PDGF-AA]

[PDGFA; PDGFA; PDGF1; PDGF-A; PDGF1; PDGF subunit A]

PDGFA_HUMAN

E Coli

Species Reactivity: Human. Cross Reactivity: Dog, Monkey, Mouse, Rat

SIEEAVPAV CKTRTVIYE IPRSQVDPT SANFLIWPP CVEVKRCTG CCNTSSVKC QPSRVHHRS VKVAKVEYV RKKPKLKEV QVRLEEHLE CACATTSLN PDYREEDTG RPRESGKKR KRKRLKPT

>=98% by SDS-PAGE gel and HPLC analyses.

Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Acetic Acid.

At -20°C

Authenticity: Verified by N-terminal and Mass Spectometry analyses (when applicable). Endotoxin: Endotoxin level is <0.1 ng/ug of protein (<1EU/ug). Protein Content: Verified by UV Spectroscopy and/or SDS-PAGE gel.

Biological Activity: Determined by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells. The expected ED 50 for this effect is 1.03-3.0 ng/ml. Reconstitution: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. For extended storage, It is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Stem Cell Research; Immunology; Cancer; Angiogenesis; Neurosciences; Bone Biology

PDGFs are disulfide-linked dimers consisting of two 12.0-13.5 kDa polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs; PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet alpha -granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR- alpha and PDGFR-beta. PDGFR- alpha is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-beta interacts with only PDGF-BB and PDGF-AB. Recombinant human PDGF-AA is a 28.5 kDa disulfide-linked homodimer of two A chains (250 total amino acids).

129719

P04085.1

P04085

Adaptive Immune System Pathway (366160); Ceramide Signaling Pathway (138023); Constitutive PI3K/AKT Signaling In Cancer Pathway (685535); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Cytokines And Inflammatory Response Pathway (198794); DAP12 Interactions Pathway (685549); DAP12 Signaling Pathway (685550); Disease Pathway (530764); Downstream Signal Transduction Pathway (106385)

The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer or as a heterodimer with the platelet-derived growth factor beta polypeptide, where the dimers are connected by disulfide bonds. Studies using knockout mice have shown cellular defects in oligodendrocytes, alveolar smooth muscle cells, and Leydig cells in the testis; knockout mice die either as embryos or shortly after birth. Two splice variants have been identified for this gene. [provided by RefSeq, Jul 2008]

PDGFA: Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal lung alveolar septum formation during embryogenesis, normal development of the gastrointestinal tract, normal development of Leydig cells and spermatogenesis. Required for normal oligodendrocyte development and normal myelination in the spinal cord and cerebellum. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFB. Belongs to the PDGF/VEGF growth factor family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Cell cycle regulation; Cytokine; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 7p22. Cellular Component: Golgi membrane; extracellular space; microvillus; cell surface; endoplasmic reticulum lumen; extracellular region. Molecular Function: collagen binding; protein binding; protein homodimerization activity; growth factor activity; protein heterodimerization activity; platelet-derived growth factor binding; platelet-derived growth factor receptor binding. Biological Process: skin development; extracellular matrix organization and biogenesis; wound healing; nerve growth factor receptor signaling pathway; negative chemotaxis; platelet-derived growth factor receptor signaling pathway; cell projection biogenesis; response to estradiol stimulus; positive regulation of MAP kinase activity; positive regulation of fibroblast proliferation; regulation of smooth muscle cell migration; platelet degranulation; cell-cell signaling; hair follicle development; positive regulation of MAPKKK cascade; transforming growth factor beta receptor signaling pathway; positive regulation of cell proliferation; positive regulation of mesenchymal cell proliferation; response to wounding; angiogenesis; inner ear development; regulation of peptidyl-tyrosine phosphorylation; response to drug; epidermal growth factor receptor signaling pathway; platelet activation; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; response to retinoic acid; response to inorganic substance; negative regulation of phosphatidylinositol biosynthetic process; positive regulation of protein amino acid autophosphorylation; positive regulation of phosphoinositide 3-kinase cascade; cell activation; positive regulation of protein kinase B signaling cascade; organ morphogenesis; positive regulation of cell division; regulation of actin cytoskeleton organization and biogenesis; response to hypoxia; innate immune response; actin cytoskeleton organization and biogenesis; blood coagulation; alveolus development; positive regulation of DNA replication; positive regulation of cell migration

0.002 mg

125 USD

0.01 mg

195

0.1 mg

640

0.5 mg

1820

1 mg

2980