Anti-murine IL-1beta | MyBioSource MBS695597 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-murine IL-1beta

catalog :

MBS695597

quantity :

0.05 mg

price :

185 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

mouse

application :

western blot, ELISA, immunohistochemistry, neutralization, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS695597

products type :

Antibody

products full name :

Anti-murine IL-1beta

products short name :

[IL-1beta]

products name syn :

[Anti-Murine IL-1beta]

other names :

[Interleukin-1 beta; Interleukin-1 beta; interleukin-1 beta; IL-1 beta; interleukin 1 beta]

products gene name :

[IL-1beta]

other gene names :

[Il1b; Il1b; Il-1b; IL-1beta; IL-1 beta]

uniprot entry name :

IL1B_MOUSE

clonality :

Polyclonal

isotype :

Rabbit IgG

host :

Rabbit

reactivity :

Mouse

form :

A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.

storage stability :

At -20 degree C

tested application :

Immunohistochemistery (IHC), Neutralization, ELISA (EIA), Western Blot (WB)

app notes :

Immunohistochemistery: This antibody stained colchicine injected mouse brain (polymorph layer of the dentate gyrus) tissue. The primary antibody was incubated at 0.5 ug/ml overnight at 4 degree C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA ) reagent. Information and photo are courtesy of the Tissue Profiling group, SciLifeLab Stockholm. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mIL-1beta (10 pg/ml), a concentration of 0.57-0.86 ug/ml of this antibody is required. Sandwich ELISA: To detect mIL-1beta by sandwich ELISA (using 100 mul/well antibody solution) a concentration of 0.5 - 2.0 mug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine IL-1beta as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-1beta. Western Blot: To detect mIL-1beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-1beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. Optimal dilutions should be determined by each laboratory for each application.

other info1 :

Immunogen: Recombinant Murine IL-1beta

other info2 :

Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. Immunostaining: This antibody stained colchicine injected mouse brain (polymorph layer of the dentate gyrus) tissue. The primary antibody was incubated at 0.5 ug/ml overnight at 4°C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA TM) reagent. Optimal concentrations and conditions may vary.

products description :

Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mIL-1beta. Anti-Murine IL-1beta specific antibody was purified by affinity chromatography employing immobilized mIL-1beta matrix.

ncbi gi num :

124304

ncbi acc num :

P10749.1

uniprot acc num :

P10749

ncbi mol weight :

150 kDa

ncbi pathways :

African Trypanosomiasis Pathway (194387); African Trypanosomiasis Pathway (194323); Alzheimer's Disease Pathway (83294); Alzheimer's Disease Pathway (509); Amoebiasis Pathway (167330); Amoebiasis Pathway (167191); Apoptosis Pathway (83257); Apoptosis Pathway (470); Chagas Disease (American Trypanosomiasis) Pathway (147810); Chagas Disease (American Trypanosomiasis) Pathway (147795)

ncbi summary :

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1. The encoded protein plays a role in thymocyte proliferation and is involved in the inflammatory response. [provided by RefSeq, Aug 2015]

uniprot summary :

IL1B: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. Monomer. Belongs to the IL-1 family. Protein type: Cytokine. Cellular Component: extracellular space; cell; extracellular region; intracellular; cytosol; vesicle; secretory granule. Molecular Function: protein domain specific binding; interleukin-1 receptor binding; cytokine activity; receptor binding. Biological Process: positive regulation of granulocyte macrophage colony-stimulating factor production; negative regulation of MAP kinase activity; positive regulation of JNK activity; positive regulation of nitric oxide biosynthetic process; negative regulation of glutamate secretion; activation of MAPK activity; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; positive regulation of interleukin-2 biosynthetic process; germ cell programmed cell death; negative regulation of insulin receptor signaling pathway; positive regulation of glial cell differentiation; positive regulation of lipid catabolic process; response to lipopolysaccharide; positive regulation of NF-kappaB import into nucleus; fever; positive regulation of membrane protein ectodomain proteolysis; response to carbohydrate stimulus; activation of NF-kappaB transcription factor; elevation of cytosolic calcium ion concentration; positive regulation of phagocytosis; positive regulation of T cell proliferation; positive regulation of astrocyte differentiation; neutrophil chemotaxis; positive regulation of heterotypic cell-cell adhesion; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of mitosis; interleukin-1 beta production; positive regulation of interleukin-6 production; social behavior; positive regulation of angiogenesis; negative regulation of neuron differentiation; positive regulation of cell division; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; negative regulation of lipid metabolic process; leukocyte migration; sequestering of triacylglycerol; positive regulation of interleukin-6 biosynthetic process; positive regulation of JNK cascade; negative regulation of transcription from RNA polymerase II promoter; positive regulation of stress-activated MAPK cascade; negative regulation of neurogenesis; positive regulation of interleukin-8 production; negative regulation of cell proliferation; negative regulation of lipid catabolic process; hyaluronan biosynthetic process; lipopolysaccharide-mediated signaling pathway; protein kinase B signaling cascade; regulation of I-kappaB kinase/NF-kappaB cascade; inflammatory response; aging; cytokine and chemokine mediated signaling pathway; MAPKKK cascade; positive regulation of immature T cell proliferation in the thymus; response to ATP; memory; positive regulation of interferon-gamma production; positive regulation of chemokine biosynthetic process; positive regulation of prostaglandin secretion; positive regulation of fever; immune response; positive regulation of protein amino acid phosphorylation; regulation of insulin secretion

size1 :

0.05 mg

price1 :

185 USD

size2 :

0.1 mg

price2 :

215

more info or order :

MyBioSource product webpage