catalog number :
MBS695543
products full name :
Biotinylated Anti-mIL-17A
products short name :
[IL-17A]
products name syn :
[Biotinylated Anti-Murine IL-17A]
other names :
[Interleukin-17A; Interleukin-17A; interleukin-17A; cytotoxic T-lymphocyte-associated antigen 8; interleukin 17A; Cytotoxic T-lymphocyte-associated antigen 8; CTLA-8]
products gene name :
[IL-17A]
other gene names :
[Il17a; Il17a; Il17; Ctla8; IL-17; Ctla-8; IL-17A; Ctla8; Il17; IL-17; IL-17A; CTLA-8]
uniprot entry name :
IL17_MOUSE
storage stability :
Store at -20°C.
tested application :
ELISA (EIA), Western Blot (WB). Optimal dilutions should be determined by each laboratory for each application.
app notes :
Direct ELISA: . To detect Murine IL-17 by direct ELISA (using 100 ul/well) a concentration of approximately 1.0ug/ml of this antibody is required. This biotinylated polyclonal antibody allows the detection of at least 0.2-0.4 ng/well of recombinant Murine-17. Sandwich ELISA: . To detect Murine IL-17 by sandwich ELISA (using 100 ul/well) a concentration of 0.25-1.0 ug/ml of this antiboy is required. The biotinylated polyclonal antibody, in conjunction with MyBioSource, Inc.'s Polyclonal Anti- Murine IL-17 (MBS695388) as a capture antibody allows the detection at least 0.2-0.4 ng/well of recombinant Murine IL-17. Western Blot: . To detect Murine IL-17A by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 mug/ml. Used in conjunction with compatible development reagents the detection limit for recombinant Murine IL-17A is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
other info1 :
Formulation: A sterile filtered antibody solution was lyophilized from PBS, pH 7.2. Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Immunogen: E.coli derived Recombinant Murine IL-17 (MBS695950)
products description :
Produced from sera of rabbits immunized with highly pure recombinant Murine IL-17. Anti-Murine IL-17 specific antibody was purified by affinity chromatography and then biotinylated.
ncbi mol weight :
Approximately 150 kDa
ncbi pathways :
Cytokine-cytokine Receptor Interaction Pathway (83248); Cytokine-cytokine Receptor Interaction Pathway (460); Inflammatory Bowel Disease (IBD) Pathway (842778); Inflammatory Bowel Disease (IBD) Pathway (842797); Rheumatoid Arthritis Pathway (200310); Rheumatoid Arthritis Pathway (200269)
uniprot summary :
IL17A: Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of ICAM1/intracellular adhesion molecule 1 in fibroblasts. Belongs to the IL-17 family. Protein type: Secreted; Secreted, signal peptide. Cellular Component: extracellular space; cytoplasm; extracellular region; external side of plasma membrane. Molecular Function: cytokine activity. Biological Process: positive regulation of interleukin-23 production; positive regulation of osteoclast differentiation; positive regulation of transcription from RNA polymerase II promoter; inflammatory response