antibody

Anti-human IL-6

MBS695447

0.05 mg

185 USD

polyclonal

rabbit

nonconjugated

western blot, ELISA, immunohistochemistry, neutralization, enzyme immunoassay, immunohistochemistry - paraffin section

Antibody

Anti-human IL-6

[IL-6]

[Anti-Human IL-6]

[Interleukin-6; Interleukin-6; interleukin-6; CDF; BSF-2; IFN-beta-2; interferon beta-2; interleukin BSF-2; interferon, beta 2; hybridoma growth factor; CTL differentiation factor; B-cell stimulatory factor 2; B-cell differentiation factor; interleukin 6; B-cell stimulatory factor 2; BSF-2; CTL differentiation factor; CDF; Hybridoma growth factor; Interferon beta-2; IFN-beta-2]

[IL-6]

[IL6; IL6; HGF; HSF; BSF2; IL-6; IFNB2; IFNB2; IL-6; BSF-2; CDF; IFN-beta-2]

IL6_HUMAN

Polyclonal

IgG

Rabbit

Human

A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.

Store at -20°C.

Immunohistochemistry (IHC) Formalin/Paraffin, Neutralization, ELISA (EIA), Western Blot (WB) . Optimal dilutions should be determined by each laboratory for each application.

Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human cervical squamous cell carcinoma. The recommended concentrations are 0.25 mug/ml-0.5 mug/ml with an overnight incubation at 4 degree C. An HRP-labeled polymer detection system was used with a DAB chromogen. The recommended antigen retrieval is heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer. *Additional Immunostaining data available. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human IL-6 (1.0 ng/ml), a concentration of 0.20 - 0.40 ug/ml of this antibody is required. Sandwich ELISA: To detect Human IL-6 by sandwich ELISA (using 100 mul/well antibody solution) a concentration of 0.5 - 2.0 mug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-6 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL-6. Western Blot: To detect Human IL-6 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IL-6 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Immunogen: E.coli derived recombinant Human IL-6 ( MBS696006 ). Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.

Produced from sera of rabbits pre-immunized with highly pure recombinant Human IL-6. Anti-Human IL-6 specific antibody was purified by affinity chromatography employing immobilized Human IL-6 matrix.

124347

P05231.1

P05231

Approximately 150 kDa

ATF-2 Transcription Factor Network Pathway (138006); Adipogenesis Pathway (198832); African Trypanosomiasis Pathway (194384); African Trypanosomiasis Pathway (194323); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Cellular Senescence Pathway (905991); Cellular Responses To Stress Pathway (645258); Chagas Disease (American Trypanosomiasis) Pathway (147809); Chagas Disease (American Trypanosomiasis) Pathway (147795)

This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. [provided by RefSeq, Jun 2011]

IL6: Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig- secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ). An inflammatory articular disorder with systemic- onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis. A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men. Belongs to the IL-6 superfamily. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 7p21. Cellular Component: extracellular space; cytoplasm; extracellular region; interleukin-6 receptor complex; external side of plasma membrane. Molecular Function: protein binding; interleukin-6 receptor binding; growth factor activity; cytokine activity. Biological Process: positive regulation of nitric oxide biosynthetic process; positive regulation of apoptosis; negative regulation of collagen biosynthetic process; positive regulation of transcription, DNA-dependent; negative regulation of cytokine secretion; response to glucocorticoid stimulus; positive regulation of JAK-STAT cascade; positive regulation of leukocyte chemotaxis; bone remodeling; glucose homeostasis; activation of NF-kappaB transcription factor; response to caffeine; positive regulation of tyrosine phosphorylation of Stat3 protein; muscle maintenance; response to antibiotic; monocyte chemotaxis; regulation of cell shape; positive regulation of T-helper 2 cell differentiation; positive regulation of acute inflammatory response; negative regulation of gluconeogenesis; acute-phase response; positive regulation of T cell proliferation; response to electrical stimulus; cell growth; defense response to virus; neurite development; response to drug; platelet activation; positive regulation of protein import into nucleus, translocation; positive regulation of interleukin-6 production; negative regulation of fat cell differentiation; response to amino acid stimulus; defense response to protozoan; positive regulation of chemokine production; defense response to Gram-positive bacterium; positive regulation of peptidyl-tyrosine phosphorylation; response to heat; cell redox homeostasis; positive regulation of B cell activation; negative regulation of protein kinase activity; neutrophil apoptosis; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; response to calcium ion; positive regulation of epithelial cell proliferation; negative regulation of apoptosis; negative regulation of muscle development; positive regulation of translation; positive regulation of smooth muscle cell proliferation; negative regulation of hormone secretion; neutrophil mediated immunity; regulation of circadian sleep/wake cycle, non-REM sleep; negative regulation of caspase activity; defense response to Gram-negative bacterium; response to insulin stimulus; negative regulation of cell proliferation; positive regulation of MAPKKK cascade; response to peptidoglycan; positive regulation of cell proliferation; hepatic immune response; inflammatory response; negative regulation of chemokine biosynthetic process; aging; response to nutrient levels; cytokine and chemokine mediated signaling pathway; positive regulation of immunoglobulin secretion; endocrine pancreas development; positive regulation of peptidyl-serine phosphorylation; regulation of angiogenesis; humoral immune response; positive regulation of osteoblast differentiation; positive regulation of protein kinase B signaling cascade; response to cold; positive regulation of neuron differentiation; positive regulation of DNA replication; positive regulation of transmission of nerve impulse. Disease: Rheumatoid Arthritis, Systemic Juvenile; Arteriovenous Malformations Of The Brain; Kaposi Sarcoma, Susceptibility To; Inflammatory Bowel Disease 1

0.05 mg

185 USD

0.1 mg

215