Biotinylated Anti-hsTRAIL / Apo2L | MyBioSource MBS695365 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Biotinylated Anti-hsTRAIL / Apo2L

catalog :

MBS695365

quantity :

0.025 mg

price :

185 USD

clonality :

polyclonal

host :

rabbit

conjugate :

biotin

reactivity :

human

application :

western blot, ELISA, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS695365

products type :

Antibody

products full name :

Biotinylated Anti-hsTRAIL / Apo2L

products short name :

[TRAIL / Apo2L]

products name syn :

[Biotinylated Anti-Human sTRAIL/Apo2L]

other names :

[Tumor necrosis factor ligand superfamily member 10; Tumor necrosis factor ligand superfamily member 10; tumor necrosis factor ligand superfamily member 10; Apo-2 ligand; TNF-related apoptosis inducing ligand TRAIL; tumor necrosis factor (ligand) family, member 10; chemokine tumor necrosis factor ligand superfamily member 10; tumor necrosis factor apoptosis-inducing ligand splice variant delta; tumor necrosis factor (ligand) superfamily, member 10; Apo-2 ligand; Apo-2L; TNF-related apoptosis-inducing ligand; Protein TRAIL; CD_antigen: CD253]

products gene name :

[sTRAIL]

products gene name syn :

[Apo2L]

other gene names :

[TNFSF10; TNFSF10; TL2; APO2L; CD253; TRAIL; Apo-2L; APO2L; TRAIL; Apo-2L; Protein TRAIL]

uniprot entry name :

TNF10_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human

form :

A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.

storage stability :

At -20 degree C

tested application :

ELISA (EIA), Western Blot (WB)

app notes :

Direct Detection: To detect Human sTRAIL/Apo2L by direct ELISA (using 100 mul/well antibody solution) a concentration of 0.25 - 1.0 mug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human sTRAIL/Apo2L. Sandwich ELISA: To detect Human sTRAIL/Apo2L by sandwich ELISA (using 100 mul/well antibody solution) a concentration of 0.25 - 1.0 mug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human sTRAIL/Apo2L as a capture antibody, allows the detection of at least 2000-4000 pg/ml of recombinant Human sTRAIL/Apo2L. Western Blot: To detect Human sTRAIL/Apo2L by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human sTRAIL/Apo2L is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Optimal dilutions should be determined by each laboratory for each application.

other info1 :

Immunogen: E.coli-derived-19.6 kDa-Recombinant-Human sTRAIL/Apo2L. Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.

other info2 :

Endotoxin: <0.1 ng/ug of protien (<1EU/ug).

products description :

Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant Human sTRAIL/Apo2L. Anti-Human sTRAIL/Apo2L specific antibody was purified by affinity chromatography and then biotinylated.

ncbi gi num :

1730015

ncbi acc num :

P50591.1

uniprot acc num :

P50591

ncbi mol weight :

~150 kDa

ncbi pathways :

Apoptosis Pathway (198797); Apoptosis Pathway (83060); Apoptosis Pathway (470); Apoptosis Pathway (105648); Apoptosis Modulation And Signaling Pathway (198822); Caspase-8 Activation By Cleavage Pathway (105654); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Death Receptor Signalling Pathway (105650); Dimerization Of Procaspase-8 Pathway (105655)

ncbi summary :

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. This protein binds to several members of TNF receptor superfamily including TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly also to TNFRSF11B/OPG. The activity of this protein may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis. The binding of this protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]

uniprot summary :

TRAIL: Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis. Homotrimer. Widespread; most predominant in spleen, lung and prostate. Belongs to the tumor necrosis factor family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Cytokine. Chromosomal Location of Human Ortholog: 3q26. Cellular Component: extracellular space; integral to plasma membrane; extracellular region. Molecular Function: protein binding; metal ion binding; cytokine activity; tumor necrosis factor receptor binding; receptor binding. Biological Process: caspase activation; positive regulation of I-kappaB kinase/NF-kappaB cascade; cell-cell signaling; positive regulation of apoptosis; apoptosis; male gonad development; positive regulation of caspase activity; immune response; signal transduction; response to insulin stimulus

size1 :

0.025 mg

price1 :

185 USD

size2 :

0.05 mg

price2 :

215

size3 :

1 mg

price3 :

2780

more info or order :

MyBioSource product webpage