Mouse Anti-Human ROBO1 | MyBioSource MBS692366 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Mouse Anti-Human ROBO1

catalog :

MBS692366

quantity :

0.1 mg

price :

490 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[14Q9]

reactivity :

human

application :

western blot, immunocytochemistry, flow cytometry

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS692366

products type :

Antibody

products full name :

Mouse Anti-Human ROBO1

products short name :

[ROBO1]

products name syn :

[Roundabout homolog 1, Deleted in U twenty twenty, H-Robo-1, ROBO1, DUTT1]

other names :

[roundabout homolog 1 isoform d; Roundabout homolog 1; roundabout homolog 1; roundabout guidance receptor 1; Deleted in U twenty twenty; H-Robo-1]

products gene name :

[ROBO1]

other gene names :

[ROBO1; ROBO1; SAX3; DUTT1; DUTT1]

clonality :

Monoclonal

isotype :

IgG2

clone :

[14Q9]

host :

Mouse

reactivity :

Human

sequence length :

1551

purity :

Protein G chromatography

form :

Lyophilized

storage stability :

Lyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least for six months without detectable loss of activity. Ships in Blue Ice.

tested application :

Western Blot (WB), Immunocytochemistry (ICC), Flow Cytometry (FC).

app notes :

Western Blot (WB) 1:1000-2000. Immunocytochemistry (ICC) 1:50-200. Flow Cytometry (FC): Yes.

other info1 :

Reconstitution: Reconstitute the antibody with 500 ul sterile PBS and the final concentration is 200 ug/ml. Antigen: Recombinant human ROBO1. Buffer: PBS

other info2 :

Remarks: This antibody recognizes human ROBO1 in direct ELISA (EIA) and Western Blot (WB).

products description :

This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells) from a mouse immunized with Recombinant human ROBO1.

ncbi gi num :

224994173

ncbi acc num :

NM_002941.3

ncbi gb acc num :

NM_001145845.1

uniprot acc num :

Q9Y6N7

ncbi pathways :

Activation Of Rac Pathway (1270321); Axon Guidance Pathway (83065); Axon Guidance Pathway (476); Axon Guidance Pathway (1270303); Developmental Biology Pathway (1270302); Inactivation Of Cdc42 And Rac Pathway (1270320); Netrin-1 Signaling Pathway (1270314); Regulation Of Commissural Axon Pathfinding By Slit And Robo Pathway (1270319); Role Of Abl In Robo-Slit Signaling Pathway (1270322); Signaling By Robo Receptor Pathway (1270318)

ncbi summary :

Bilateral symmetric nervous systems have special midline structures that establish a partition between the two mirror image halves. Some axons project toward and across the midline in response to long-range chemoattractants emanating from the midline. The product of this gene is a member of the immunoglobulin gene superfamily and encodes an integral membrane protein that functions in axon guidance and neuronal precursor cell migration. This receptor is activated by SLIT-family proteins, resulting in a repulsive effect on glioma cell guidance in the developing brain. A related gene is located at an adjacent region on chromosome 3. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

uniprot summary :

ROBO1: Receptor for SLIT1 and SLIT2 which are thought to act as molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex. May be required for lung development. Belongs to the immunoglobulin superfamily. ROBO family. 5 isoforms of the human protein are produced by alternative splicing. Protein type: Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 3p12.3. Cellular Component: cell surface; cytoplasm; integral to plasma membrane; plasma membrane. Molecular Function: axon guidance receptor activity; identical protein binding; LRR domain binding; protein binding. Biological Process: axon guidance; axon midline choice point recognition; caspase activation; cell adhesion; cell migration during sprouting angiogenesis; chemorepulsion involved in postnatal olfactory bulb interneuron migration; homophilic cell adhesion; negative regulation of cell migration; negative regulation of mammary gland epithelial cell proliferation; negative regulation of negative chemotaxis; nervous system development; positive regulation of axonogenesis; positive regulation of Rho protein signal transduction

size1 :

0.1 mg

price1 :

490 USD

more info or order :

MyBioSource product webpage