Mouse Anti-Human NRP-2 | MyBioSource MBS692353 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Mouse Anti-Human NRP-2

catalog :

MBS692353

quantity :

0.1 mg

price :

490 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[29H31]

reactivity :

human

application :

western blot

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS692353

products type :

Antibody

products full name :

Mouse Anti-Human NRP-2

products short name :

[NRP-2]

products name syn :

[NRP-2; Vascular endothelial cell growth factor 165 receptor 2; VEGF165R2]

other names :

[neuropilin-2 isoform 2; Neuropilin-2; neuropilin-2; neuropilin 2; Vascular endothelial cell growth factor 165 receptor 2]

products gene name :

[NRP-2]

other gene names :

[NRP2; NRP2; NP2; NPN2; PRO2714; VEGF165R2; VEGF165R2]

clonality :

Monoclonal

isotype :

IgG2

clone :

[29H31]

host :

Mouse

reactivity :

Human

sequence length :

926

purity :

Protein G chromatography

form :

Lyophilized

storage stability :

Lyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20 °C for at least for six months without detectable loss of activity.

tested application :

Western Blot (WB)

app notes :

WB: 1:1000-2000

other info1 :

Reconstitution: Reconstitute the antibody with 500 ul sterile PBS and the final concentration is 200 ug/ml. Antigen: Recombinant Human Neuropilin-2. Reconstitution Buffer: PBS

other info2 :

Remarks: This antibody recognizes human Neuropilin-2 in direct ELISAs and WBs. Preparation: This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse immunized with human Neuropilin-2 recombinant protein). The IgG1 fraction of culture supernatant was purified by Protein G affinity chromatography.

products description :

Neuropilin receptor 2 (NRP-2) is a 120 kDa, type I transmembrane (TM) glycoprotein that is related to the semaphorin receptor family. NRP-2 is a complex molecule with multiple splice forms. Five transmembrane forms are known, and one 62 kDa soluble form has been identified. Based on the originally reported precursor size of 909 amino acids (aa), the œStandard precursor in human will have a 20 aa signal sequence, an 842 aa extracellular region, a 25 aa TM segment, and a 42 aa cytoplasmic tail. The extracellular region contains two N terminal CUB domains, two jellyroll shaped coagulation factor V type C domains, and a juxtamembrane MAM domain. The CUB and factor V domain are involved in VEGF and semaphorin binding. The MAM domain appears necessary for signaling through Plexin. The five transmembrane isoforms all share the same CUB, factor V and MAM domains. Two of the four variants show a complete replacement of these 101 aa with a totally unrelated stretch of approximately 90 aa. This creates a new TM and cytoplasmic tail. These forms are called œNRP 2b forms. Two other isoforms (plus the standard 909 aa form) retain the 101 aa stretch, and add either 17 or 22 aa to the end of the extracellular region. These forms are called œNRP 2a forms. The soluble form of NRP 2 is 555 aa in precursor length, and contains the two CUB domains plus the first 1½ factor V type C domains. NRP 2 binds Sema3B through F, and VEGF isoforms 165, 145, PlGF-2 and VEGF-C. It is known to form homodimers and heterodimers with NRP 1, and it forms receptor complexes with Plexin 1 and VEGFR-1. NRP 2 is found on a variety of cell types including neurons, vascular endothelial cells, Schwann cells and pancreatic acinar cells.

ncbi gi num :

41872533

ncbi acc num :

NP_003863.2

ncbi gb acc num :

NM_003872.2

uniprot acc num :

O60462-6

ncbi pathways :

Axon Guidance Pathway (1270303); Developmental Biology Pathway (1270302); L1CAM Interactions Pathway (1270323); Neurophilin Interactions With VEGF And VEGFR Pathway (1269486); NrCAM Interactions Pathway (1270329); Signal Transduction Pathway (1269379); Signaling By VEGF Pathway (1269480); Signaling Events Mediated By VEGFR1 And VEGFR2 Pathway (137940); VEGFR1 Specific Signals Pathway (137924)

ncbi summary :

This gene encodes a member of the neuropilin family of receptor proteins. The encoded transmembrane protein binds to SEMA3C protein {sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3C} and SEMA3F protein {sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F}, and interacts with vascular endothelial growth factor (VEGF). This protein may play a role in cardiovascular development, axon guidance, and tumorigenesis. Multiple transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

High affinity receptor for semaphorins 3C, 3F, VEGF-165 and VEGF-145 isoforms of VEGF, and the PLGF-2 isoform of PGF.

size1 :

0.1 mg

price1 :

490 USD

more info or order :

MyBioSource product webpage