protein

Rat Podoplanin, soluble

MBS692151

0.005 mg

180 USD

Recombinant Protein

Rat Podoplanin, soluble

Podoplanin, soluble

Recombinant Rat soluble Podoplanin; Gp38; Glycoprotein 38; Ots8; T1-alpha

podoplanin; Podoplanin; podoplanin; T1A; RTI140; glycoprotein 38; E11 antigen epitope; type I cell 40 kDa protein; epithelial cell surface transmembrane protein antigen; pulmonary type I alveolar epithelial cell transmembrane differentiation marker; podoplanin; E11 antigen epitope; Glycoprotein 38; Gp38; OTS-8; RTI140; T1-alpha; T1A; Type I cell 40 kDa protein

Pdpn; Pdpn; E11; Gp38; OTS-8; RTI40; T1-alpha; Gp38; Ots8; Gp38; T1A

PDPN_RAT

E Coli

Rat

120

GAIGALEDDLVTPGPGDDMVNPGLEDRIETTDTTGELDK
STAKAPLVPTQPPIEELPTSGTSDHDHKEHESTTTVKAV
TSHSTDKKTTHPNRDNAGGETQTTDKKDGLAVVTLEHHH
HHH

> 98% by SDS-PAGE & silver stain

Lyophilized

Stability: The lyophilized protein is stable for a few weeks at room temperature, but best stored at –20 degree C. Reconstituted soluble Podoplanin should be stored in working aliquots at –20 degree C. Reconstitution: We recommend a quick spin followed by reconstitution in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4 degree C for 1 week or -20 degree C for future use. Avoid freeze/thaw cycles.

N Terminal Sequence: GAIGALED

Buffer: 0.5x PBS. Biological Activity: Not tested so far.

Soluble Receptors

Podoplanin, also known as glycoprotein 38 (gp38), PA2.26 antigen, T1alpha (T1A), and aggrus, is a 38 kDa type I transmembrane sialoglycoprotein and member of the podoplanin family. Podoplanin is synthesized as a 172 amino acid (aa) precursor with a 22 aa signal sequence, a 119 aa extracellular domain (ECD), a 21 aa transmembrane region, and a short, 10 aa cytoplasmic tail. The ECD contains abundant Ser/Thr residues as potential sites for Oglycosylation, and the cytoplasmic region contains putative sites for kinase C and cAMP phosphorylation. Mouse Podoplanin shares 77% and 46% aa sequence identity with rat and human Podoplanin, respectively. Podoplanin is expressed on glomerular epithelial cells (podocytes), type I lung alveolar cells, lymphatic endothelial cells, and on numerous tumors including colorectal tumors, squamous cell carcinomas, testicular seminoma, and brain tumors. One study shows high expression of Podoplanin mRNA in placenta, lung, skeletal muscle, and heart, and weaker levels in brain, kidney, and liver. Podoplanin is the ligand for Ctype lectin like receptor 2 (CLEC2). Their association is dependent on sialic acid on Oglycans of Podoplanin. Through its association with CLEC2, Podoplanin induces platelet aggregation and tumor metastasis. Podoplanin is also necessary for lymphatic vessel formation, normal lung cell proliferation and alveolus formation at birth.

1. Zimmer et al, Biochem J 341:277, 1999 2. KatsueInoue et al, J Biol Chem 282:25993, 2007 3. Kato et al, J Biol Chem 278:51599, 2003 4. Schacht et al, Am J Pathol 166:913, 2005 5. Breiteneder-Geleff et al, Am J Pathol 151:1141, 1997 6. Kato et al, Tumour Biol 26:195, 2005 7. Kato et al, Oncogene 23:8552, 2004 8. Mishima et al, Acta Neuropathol 111:563, 2006 9. Mishima et al, Acta Neuropathol 111:483, 2006 10. Kato et al, Biochem Biophys Res Commun 349:1301, 2006

9506743

NP_062231.1

NM_019358.1

Q64294

12,8 kDa

membrane glycoprotein that may function in lung development [RGD, Feb 2006]

Function: May be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes, induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. Required for normal lung cell proliferation and alveolus formation at birth. Induces platelet aggregation. Does not have any effect on folic acid or amino acid transport. Does not function as a water channel or as a regulator of aquaporin-type water channels . By similarity. Subcellular location: Membrane; Single-pass type I membrane protein. Cell projection lamellipodium membrane; Single-pass type I membrane protein. Cell projection filopodium membrane; Single-pass type I membrane protein. Cell projection microvillus membrane; Single-pass type I membrane protein. Cell projection ruffle membrane; Single-pass type I membrane protein. Note: Localized to actin-rich microvilli and plasma membrane projections such as filopodia, lamellipodia and ruffles. Ref.3. Tissue specificity: In adult kidney, expressed on the urinary surface and foot processes of podocytes and in parietal epithelial cells of Bowman's capsule where it is localized to luminal surfaces. In lung, expressed exclusively on luminal surfaces of type I alveolar epithelial cells and pleural mesothelial cells. Not expressed in type II alveolar cells. In bone, expressed in osteocytes and osteoblasts. In spleen, liver, stomach and intestine, expressed in mesoepithelium. Also expressed in thymic epithelial cells, choroid plexus and leptomeninges. Ref.3 Ref.4. Developmental stage: In newborn kidney, not detected at the vesicle stage. First detected in S-shaped bodies. Ref.3. Post-translational modification: Extensively O-glycosylated. Contains sialic acid residues. O-glycosylation is necessary for platelet aggregation activity. Ref.6The N-terminus is blocked. Miscellaneous: Down-regulated in puromycin aminonucleoside nephrosis (PAN). Sequence similarities: Belongs to the podoplanin family.

0.005 mg

180 USD