protein

Human Semaphorin 3A

MBS692128

0.025 mg

340 USD

Recombinant Protein

Human Semaphorin 3A

Semaphorin 3A

SEMA3A; SEMAD

semaphorin-3A; Semaphorin-3A; semaphorin-3A; collapsin 1; semaphorin D; semaphorin L; semaphorin III; sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A; Semaphorin III

SEMA3A; SEMA3A; HH16; SemD; COLL1; SEMA1; SEMAD; SEMAL; coll-1; Hsema-I; SEMAIII; Hsema-III; SEMAD; Sema III

SEM3A_HUMAN

CHO Cells

Human

771

NYQNGKNNVP RLKLSYKEML ESNNVITFNG LANSSSYHTF LLDEER SRLY VGAKDHIFSF DLVNIKDFQK IVWPVSYTRR DECKWAGKDI L KECANFIKV LKAYNQTHLY ACGTGAFHPI CTYIEIGHHP EDNIFKL ENS HFENGRGKSP YDPKLLTASL LIDGELYSGT AADFMGRDFA IF RTLGHHHP IRTEQHDSRW LNDPKFISAH LISESDNPED DKVYFFFR EN AIDGEHSGKA THARIGQICK NDFGGHRSLV NKWTTFLKAR LIC SVPGPNG IDTHFDELQD VFLMNFKDPK NPVVYGVFTT SSNIFKGSA V CMYSMSDVRR VFLGPYAHRD GPNYQWVPYQ GRVPYPRPGT CPSK TFGGFD STKDLPDDVI TFARSHPAMY NPVFPMNNRP IVIKTDVNYQ FTQIVVDRVD AEDGQYDVMF IGTDVGTVLK VVSIPKETWY DLEEV LLEEM TVFREPTAIS AMELSTKQQQ LYIGSTAGVA QLPLHRCDIY GKACAECCLA RDPYCAWDGS ACSRYFPTAK RRTRRQDIRN GDPLTH CSDL HHDNHHGHSP EERIIYGVEN SSTFLECSPK SQRALVYWQF Q RRNEERKEE IRVDDHIIRT DQGLLLRSLQ QKDSGNYLCH AVEHGFI QTL LKVTLEVIDT EHLEELLHKD DDGDGSKTKE MSNSMTPSQK VW YRDFMQLI NHPNLNTMDE FCEQVWKRDR KQRRQRPGHT PGNSNKWK HL QENKKGRNRR THEFERAPRS V

> 95% by SDS-PAGE & HPLC analyses

Lyophilized from 20mM Tris, pH 8.0

The lyophilized protein is stable for 1 month at room temperature and 6 months at 4 degree C, but best stored at -20 degree C. Reconstituted human Semaphorin 3A is stable for 1 week at 2 degree C to 8 degree C and 3 months at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Endotoxin level: <0.1 ng per ug of Semaphorin 3A

Biological Activity: Determined by its ability to bind recombinant rat Neuropilin-1 Fc Chimera in a functional ELISA assay. Reconstitution: Reconstituted in water to a concentration of 0.1-1.0 mg/ml. Do not vortex! For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20 degree C to -80 degree C.

Cytokines & Growth Factors

Semaphorins are a large group of structurally related secreted, GPI-anchored and transmembrane cell signaling molecules. There are 8 major classifications (1-7) of Semaphorins characterized by the existence of a conserved 500 amino acid SEMA domain at the amino terminus. Classes 3,4,6, and 7 are found in vertebrates only, whilst class 5 is found in both vertebrates and invertebrates. Each class is then divided into additional subgroups based on shared structural characteristics. Semaphorins primarily function as axon growth cone guidance factors during neuronal development. Semaphorin 3A acts as a chemo-repellent to axons, and an inhibitor of the growth of axons by signaling through receptors, Neuropilin-1and Plexin-A. Recombinant human Semaphorin 3A is a 751 amino acid protein containing the SEMA domain, an immunoglobulin c2-like domain and a basic domain (Arg/Lys rich) near the C-terminus.

5174673

NP_006071.1

NM_006080.2

Q14563

~85kDa

Axon Guidance Pathway (83065); Axon Guidance Pathway (476); Axon Guidance Pathway (105688); CRMPs In Sema3A Signaling Pathway (119525); Developmental Biology Pathway (477129); SEMA3A-Plexin Repulsion Signaling By Inhibiting Integrin Adhesion Pathway (119523); Sema3A PAK Dependent Axon Repulsion Pathway (119524); Semaphorin Interactions Pathway (119519)

This gene is a member of the semaphorin family and encodes a protein with an Ig-like C2-type (immunoglobulin-like) domain, a PSI domain and a Sema domain. This secreted protein can function as either a chemorepulsive agent, inhibiting axonal outgrowth, or as a chemoattractive agent, stimulating the growth of apical dendrites. In both cases, the protein is vital for normal neuronal pattern development. Increased expression of this protein is associated with schizophrenia and is seen in a variety of human tumor cell lines. Also, aberrant release of this protein is associated with the progression of Alzheimer's disease. [provided by RefSeq, Jul 2008]

Function: Involved in the development of the olfactory system and in neuronal control of puberty. Induces the collapse and paralysis of neuronal growth cones. Could serve as a ligand that guides specific growth cones by a motility-inhibiting mechanism. Binds to the complex neuropilin-1/plexin-1. Ref.3. Subunit structure: Interacts with PLXND1 . By similarity. Subcellular location: Secreted . By similarity. Domain: Strong binding to neuropilin is mediated by the carboxy third of the protein. Involvement in disease: Hypogonadotropic hypogonadism 16 with or without anosmia (HH16) [MIM:614897]: A disorder characterized by absent or incomplete sexual maturation by the age of 18 years, in conjunction with low levels of circulating gonadotropins and testosterone and no other abnormalities of the hypothalamic-pituitary axis. In some cases, it is associated with non-reproductive phenotypes, such as anosmia, cleft palate, and sensorineural hearing loss. Anosmia or hyposmia is related to the absence or hypoplasia of the olfactory bulbs and tracts. Hypogonadism is due to deficiency in gonadotropin-releasing hormone and probably results from a failure of embryonic migration of gonadotropin-releasing hormone-synthesizing neurons. In the presence of anosmia, idiopathic hypogonadotropic hypogonadism is referred to as Kallmann syndrome, whereas in the presence of a normal sense of smell, it has been termed normosmic idiopathic hypogonadotropic hypogonadism (nIHH).Note: The disease may be caused by mutations affecting distinct genetic loci, including the gene represented in this entry. Ref.3 Ref.6. Sequence similarities: Belongs to the semaphorin family.Contains 1 Ig-like C2-type (immunoglobulin-like) domain.Contains 1 PSI domain.Contains 1 Sema domain.

0.025 mg

340 USD