protein

Human Angiopoietin-2 / Ang-2

MBS691998

0.005 mg

200 USD

Recombinant Protein

Human Angiopoietin-2 / Ang-2

[Angiopoietin-2 / Ang-2]

[ANGPT2; ANG2; AGPT2]

[versican core protein isoform 2; Versican core protein; versican core protein; versican proteoglycan; large fibroblast proteoglycan; glial hyaluronate-binding protein; chondroitin sulfate proteoglycan 2; chondroitin sulfate proteoglycan core protein 2; versican; Chondroitin sulfate proteoglycan core protein 2; Chondroitin sulfate proteoglycan 2; Glial hyaluronate-binding protein; GHAP; Large fibroblast proteoglycan; PG-M]

[Ang-2]

[VCAN; VCAN; WGN; ERVR; GHAP; PG-M; WGN1; CSPG2; CSPG2; Chondroitin sulfate proteoglycan 2; GHAP]

CSPG2_HUMAN

CHO Cells

Human

435

DAPLEYDDSV QRLQVLENIM ENNTQWLMKL ENYIQDNMKK EMVEIQ QNAV QNQTAVMIEI GTNLLNQTAE QTRKLTDVEA QVLNQTTRLE L QLLEHSLST NKLEKQILDQ TSEINKLQDK NSFLEKKVLA MEDKHII QLQ SIKEEKDQLQ VLVSKQNSII EELEKKIVTA TVNNSVLQKQ QH DLMETVNN LLTMMSTSNS AKDPTVAKEE QISFRDCAEV FKSGHTTN GI YTLTFPNSTE EIKAYCDMEA GGGGWTIIQR REDGSVDFQR TWK EYKVGFG NPSGEYWLGN EFVSQLTNQQ RYVLKIHLKD WEGNEAYSL Y EHFYLSSEEL NYRIHLKGLT GTAGKISSIS QPGNDFSTKD GDND KCICKC SQMLTGGWWF DACGPSNLNG MYYPQRQNTN KFNGIKWYYW KGSGYSLKAT TMMIRPADFH HHHHH

> 95%, by SDS-PAGE and HPLC analysis

Lyophilized

The lyophilized protein is stable at room temperature for 1 month and at 4°C for 3 months. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 3 months at -20°C to -80°C. Avoid repeated freeze and thaw cycles!

Length (aa): 435. Gene-ID: 285. mRNA RefSeq: NM_001147.2. Endotoxin Level: < 0.1 ng/ug of protein (<1EU/ug). Reconstitution: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Tag Information: His-Tag. Biological Activity: Determined by its ability to stimulate tubulogenesis in HUVEC cells using concentration of 0.2ug/ml.

Cytokines & Growth Factors

Human Angiopoitin-2 (Ang-2), a 66 kDa protein consisting of 476 amino acid residues (N21-F496), is fused to a N-terminal myc-tag and produced in insect cells. The angiopoietin (Ang) family of growth factors includes four members, all of which bind to the endothelial receptor tyrosine kinase Tie2. Two of the Angs, Ang-1 and Ang-4, activate the Tie2 receptor, whereas Ang-2 and Ang-3 inhibit Ang-1-induced Tie2 phosphorylation. Angiopoietin-1 (Ang-1) is a secreted growth factor which binds to and activates the Tie-2 receptor tyrosine kinase. The factor enhances endothelial cell survival and capillary morphogenesis, and also limits capillary permeability. Ang-2 binds the same receptor but fails to activate it: hence, it is a natural inhibitor of Ang-1. Ang-2 destabilises capillary integrity, facilitating sprouting when ambient vascular endothelial growth factor (VEGF) levels are high, but causing vessel regression when VEGF levels are low. Tie-1 is a Tie-2 homologue but its ligands are unknown. Angiopoietin and Tie genes are expressed in the mammalian metanephros, the precursor of the adult kidney, where they may play a role in endothelial precursor growth. Tie-1-expressing cells can be detected in the metanephros when it first forms and, based on transplantation experiments, these precursors contribute to the generation of glomerular capillaries. During glomerular maturation, podocyte-derived Ang-1 and mesangial-cell-derived Ang-2 may affect growth of nascent capillaries. After birth, vasa rectae acquire their mature configuration and Ang-2 expressed by descending limbs of loops of Henle would be well placed to affect the growth of this medullary microcirculation. Finally, preliminary data implicate angiopoietins in deregulated vessel growth in Wilms ' kidney tumours and in vascular remodelling after nephrotoxicity. Altogether, existing data suggest that VEGF-A and Angiopoietins not only have quite different roles during vascular development, but also very complementary and coordinated roles.

187607300

NP_001138

NM_001126336.2

Q15123

60-70 kDa

A Tetrasaccharide Linker Sequence Is Required For GAG Synthesis Pathway (645305); CS/DS Degradation Pathway (645311); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Chondroitin Sulfate Biosynthesis Pathway (645309); Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway (645308); Dermatan Sulfate Biosynthesis Pathway (645310); Direct P53 Effectors Pathway (137939); Disease Pathway (530764); ECM Proteoglycans Pathway (833812)

This gene is a member of the aggrecan/versican proteoglycan family. The protein encoded is a large chondroitin sulfate proteoglycan and is a major component of the extracellular matrix. This protein is involved in cell adhesion, proliferation, proliferation, migration and angiogenesis and plays a central role in tissue morphogenesis and maintenance. Mutations in this gene are the cause of Wagner syndrome type 1. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009]

Function: May play a role in intercellular signaling and in connecting cells with the extracellular matrix. May take part in the regulation of cell motility, growth and differentiation. Binds hyaluronic acid. Subunit structure: Interacts with FBLN1 . By similarity. Subcellular location: Secreted extracellular space extracellular matrix. Tissue specificity: Cerebral white matter and plasma. Isoform V0 and isoform V1 are expressed in normal brain, gliomas, medulloblastomas, schwannomas, neurofibromas, and meningiomas. Isoform V2 is restricted to normal brain and gliomas. Isoform V3 is found in all these tissues except medulloblastomas. Ref.12. Developmental stage: Disappears after the cartilage development. Post-translational modification: Phosphorylation sites are present in the extracellular medium. Involvement in disease: Wagner vitreoretinopathy (WGVRP) [MIM:143200]: A rare vitreoretinopathy characterized by an optically empty vitreous cavity with fibrillary condensations and a preretinal avascular membrane. Other optical features include progressive chorioretinal atrophy, perivascular sheating, subcapsular cataract and myopia.Note: The disease is caused by mutations affecting the gene represented in this entry. The pathological mechanism involves a quantitave imbalance of the normally occurring splice variants (Ref.15). Ref.13 Ref.15. Sequence similarities: Belongs to the aggrecan/versican proteoglycan family.Contains 1 C-type lectin domain.Contains 2 EGF-like domains.Contains 1 Ig-like V-type (immunoglobulin-like) domain.Contains 2 Link domains.Contains 1 Sushi (CCP/SCR) domain.

0.005 mg

200 USD