protein

Human Sox2

MBS691771

0.025 mg

340 USD

Recombinant Protein

Human Sox2

Sox2

Recombinant Human Sox2

transcription factor SOX-2; Transcription factor SOX-2; transcription factor SOX-2; transcription factor SOX2; SRY-related HMG-box gene 2; SRY (sex determining region Y)-box 2

Sox2

SOX2; SOX2; ANOP3; MCOPS3

SOX2_HUMAN

E Coli

Human

317

MYNMMETELK PPGPQQTSGG GGGNSTAAAA GGNQKNSPDR VKRPMN AFMV WSRGQRRKMA QENPKMHNSE ISKRLGAEWK LLSETEKRPF I DEAKRLRAL HMKEHPDYKY RPRRKTKTLM KKDKYTLPGG LLAPGGN SMA SGVGVGAGLG AGVNQRMDSY AHMNGWSNGS YSMMQDQLGY PQ HPGLNAHG AAQMQPMHRY DVSALQYNSM TSSQTYMNGS PTYSMSYS QQ GTPGMALGSM GSVVKSEASS SPPVVTSSSH SRAPCQAGDL RDM ISMYLPG AEVPEPAAPS RLHMSQHYQS GPVPGTAING TLPLSHM

>95 % (SDS-PAGE, HPLC)

Lyophilized

The lyophilized protein is stable at room temperature for 1 month and at 4 degree C for 6 months. Reconsituted working aliquots are stable for 1 week at 2 to 8 degree C and for 3 months at -20 to -80 degree C.

Endotoxin Level: < 0.1 ng/ug of protein (<1EU/ug)

Biological Activity: Data not available. Reconstitution: Centrifuge the vial or to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/m. Do not vortex. This solution can be stored at 2-8 degree C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer (e.g. PBS) containing a carrier protein (example 0.1 BSA) and store in working aliquots at -20 to -80 degree C.

Cytokines & Growth Factors

Sox2, also known as sex determining region Y (SRY)-box 2, belongs to a diverse family of structurally-related transcription factors whose primary structure contains a 79-residue DNA-binding domain, called high mobility group (HMG) box. It plays an essential role in maintaining the pluripotency of embryonic stem cells (ESC) and determination of cell fate. Microarray analysis showed that Sox2 regulates the expression of multiple genes involved in embryonic development including FGF-4, YES1 and ZFP206. Sox2 acts as a transcriptional activator after forming a ternary complex with Oct3/4 and a conserved non-coding DNA sequence (CNS1) located approximately 2 kb upstream of the RAX promoter. The introduction of Sox2, Oct4, Myc, and Klf4, into human dermal fibroblasts isolated from a skin biopsy of a healthy research fellow was sufficient to confer a pluripotent state upon the fibroblast genome. The reprogrammed cells thus obtained resemble ESC in morphology, gene expression, and in the capacity to form teratomas in immune-deficient mice. Recombinant human Sox2 is a 34.3 kDa protein containing 317 amino-acid residues.

28195386

NP_003097.1

NM_003106.3

P48431

34.3 kDa

Cardiac Progenitor Differentiation Pathway (712094); Hippo Signaling Pathway (749777); Hippo Signaling Pathway (750388); SIDS Susceptibility Pathways (198901); Wnt Signaling Pathway And Pluripotency (198847)

This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). [provided by RefSeq, Jul 2008]

Function: Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 . By similarity. Critical for early embryogenesis and for embryonic stem cell pluripotency. May function as a switch in neuronal development. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal . By similarity. Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation . By similarity. Ref.5. Subunit structure: Interacts with ZSCAN10 . By similarity. Interacts with SOX3 and FGFR1 . By similarity. Subcellular location: Nucleus. Post-translational modification: Sumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation . By similarity. Involvement in disease: Microphthalmia, syndromic, 3 (MCOPS3) [MIM:206900]: A disease characterized by the rare association of malformations including uni- or bilateral anophthalmia or microphthalmia, and esophageal atresia with trachoesophageal fistula. Microphthalmia is a disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.4. Biotechnological use: POU5F1/OCT4, SOX2, MYC/c-Myc and KLF4 are the four Yamanaka factors. When combined, these factors are sufficient to reprogram differentiated cells to an embryonic-like state designated iPS (induced pluripotent stem) cells. iPS cells exhibit the morphology and growth properties of ES cells and express ES cell marker genes. Ref.5. Sequence similarities: Contains 1 HMG box DNA-binding domain. Sequence caution: The sequence AAA35997.1 differs from that shown. Reason: Erroneous initiation. The sequence CAA83435.1 differs from that shown. Reason: Erroneous initiation.

0.025 mg

340 USD