Mouse TNF-alpha | MyBioSource MBS691749 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Mouse TNF-alpha

catalog :

MBS691749

quantity :

0.005 mg

price :

200 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS691749

products type :

Recombinant Protein

products full name :

Mouse TNF-alpha

products short name :

[TNF-alpha]

products name syn :

[Tnf; DIF; Tnfa; TNFSF2; Tnfsf1a; TNFalpha; TNF-alpha]

other names :

[tumor necrosis factor isoform 2; Tumor necrosis factor; tumor necrosis factor; cachectin; tumor necrosis factor-alpha; tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-aCleaved into the following 6 chains:Tumor necrosis factor, membrane form; Alternative name(s):; N-terminal fragment]

products gene name :

[TNF-alpha]

other gene names :

[Tnf; Tnf; DIF; Tnfa; TNF-a; TNFSF2; Tnfsf1a; TNFalpha; TNF-alpha; Tnfa; Tnfsf2; TNF-a; NTF; ICD1; ICD2]

uniprot entry name :

TNFA_MOUSE

host :

E Coli

reactivity :

Mouse

sequence length :

156

sequence :

MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKD NQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK V NLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVN LPK YLDFAESGQV YFGVIAL

purity :

> 98% (SDS-PAGE; HPLC analyses

form :

Lyophilized

storage stability :

The lyophilized protein is stable at room temperature for 1 month and at 4 degree C for 6 months. Reconstituted working aliquots are stable for 1 week at 2 degree C to 8 degree C and for 12 months at -20 degree C to -80 degree C. Avoid freeze/thaw cycles.

other info1 :

Endotoxin level: =1 x 10^7 units/mg. Length: 156

other info2 :

Reconstitution: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/m. Do not votex. This solution can be stored at 2 degree C to 8 degree C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20 degree C to -80 degree C.

products categories :

Cytokines & Growth Factors

products description :

TNF-alpha is a pleiotropic pro-inflammatory cytokine secreted by various cells including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF family of ligands and signals through two receptors, TNFR1 and TNFR2. TNF-alpha is cytotoxic to a wide variety of tumor cells and is an essential factor in mediating the immune response against bacterial infections. TNF-alpha also plays a role in the induction of septic shock, auto immune diseases, rheumatoid arthritis, inflammation, and diabetes. Recombinant murine TNF-alpha is a soluble 156 amino acid protein (17.3 kDa) which corresponds to C-terminal extracellular domain of the full length transmembrane protein.

ncbi gi num :

518831589

ncbi acc num :

NM_013693.2

ncbi gb acc num :

NM_001278601.1

uniprot acc num :

P06804

ncbi mol weight :

17.3 kDa

ncbi pathways :

Adipocytokine Signaling Pathway (83290); Adipocytokine Signaling Pathway (505); Adipogenesis Pathway (198299); African Trypanosomiasis Pathway (194387); African Trypanosomiasis Pathway (194323); Allograft Rejection Pathway (83316); Allograft Rejection Pathway (535); Alzheimer's Disease Pathway (83294); Alzheimer's Disease Pathway (509); Amoebiasis Pathway (167330)

ncbi summary :

This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. Members of this family are classified based on primary sequence, function, and structure. This protein is synthesized as a type-II transmembrane protein and is reported to be cleaved into products that exert distinct biological functions. It plays an important role in the innate immune response as well as regulating homeostasis but is also implicated in diseases of chronic inflammation. In mouse deficiency of this gene is associated with defects in response to bacterial infection, with defects in forming organized follicular dendritic cell networks and germinal centers, and with a lack of primary B cell follicles. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]

uniprot summary :

Function: Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells . By similarity. Subunit structure: Interacts with SPPL2B . By similarity. Homotrimer. Subcellular location: Cell membrane; Single-pass type II membrane protein. Tumor necrosis factor, membrane form: Membrane; Single-pass type II membrane protein . By similarity. Tumor necrosis factor, soluble form: Secreted. C-domain 1: Secreted . By similarity. C-domain 2: Secreted . By similarity. Post-translational modification: The membrane-bound form is further proteolytically processed by SPPL2A or SPPL2B through regulated intramembrane proteolysis producing TNF intracellular domains (ICD1 and ICD2) released in the cytosol and TNF C-domain 1 and C-domain 2 secreted into the extracellular space . By similarity. The soluble form derives from the membrane form by proteolytic processing.The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1 . By similarity.O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid . By similarity. Sequence similarities: Belongs to the tumor necrosis factor family.

size1 :

0.005 mg

price1 :

200 USD

size2 :

0.02 mg

price2 :

340

more info or order :

MyBioSource product webpage