protein

Human FGF-2 (basic) 146 aa

MBS691741

0.01 mg

200 USD

Recombinant Protein

Human FGF-2 (basic) 146 aa

[FGF-2 (basic) 146 aa]

[FGF2; BFGF; FGFB; HBGF-2; basic Fibroblast growth factor (bFGF); Heparin binding growth factor-3; Prostatropin]

[fibroblast growth factor 2; Fibroblast growth factor 2; fibroblast growth factor 2; prostatropin; heparin-binding growth factor 2; basic fibroblast growth factor bFGF; fibroblast growth factor 2 (basic); Basic fibroblast growth factor; bFGF; Heparin-binding growth factor 2]

[FGF-2]

[FGF2; FGF2; BFGF; FGFB; FGF-2; HBGF-2; FGFB; FGF-2; bFGF; HBGF-2]

FGF2_HUMAN

Human

[146]

146

PALPEDGGSG AFPPGHFKDP KRLYCKNGGF FLRIHPDGRV DGVREK SDPH IKLQLQAEER GVVSIKGVCA NRYLAMKEDG RLLASKCVTD E CFFFERLES NNYNTYRSRK YTSWYVALKR TGQYKLGSKT GPGQKAI LFL PMSAKS

> 98% by SDS-PAGE & Silver stain

Lyophilized

The lyophilized protein is stable room temperature for 1 month and at 4°C for 6 months. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 3 months at -20°C to -80°C.

Expressed in: E Coli. Biological Activity: The ED 50 was determined by a cell proliferation assay using balb/c 3T3 cells is = 2 x 10 7 units/mg. Endotoxin level: < 0.1 ng/ug of protein (<1 EU/ug). Reconstitution: Centrifuge prior to opening. Reconstitute in 5mM Tris, pH 7.6 to a concentration of 0.1-1.0 mg/m. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommened to further dilute in a buffer containg a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Cytokines & Growth Factors

FGF-basic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-basic is a non-glycosylated heparin binding growth factor that is expressed in the brain, pituitary, kidney, retina, bone, testis, adrenal gland liver, monocytes, epithelial cells and endothelial cells. FGF-basic signals through FGFR 1b, 1c, 2c, 3c and 4. Recombinant Human FGF-basic (146 a.a.) is a 16.4 kDa protein consisting of 146 amino acid residues.

153285461

NP_001997.5

NM_002006.4

P09038

16.4 kDa

Activated Point Mutants Of FGFR2 Pathway (645281); Adaptive Immune System Pathway (366160); Angiogenesis Pathway (198772); Angiopoietin Receptor Tie2-mediated Signaling Pathway (137917); Cardiac Progenitor Differentiation Pathway (712094); Constitutive PI3K/AKT Signaling In Cancer Pathway (685535); DAP12 Interactions Pathway (685549); DAP12 Signaling Pathway (685550); Disease Pathway (530764); Downstream Signaling Events Of B Cell Receptor (BCR) Pathway (576250)

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. [provided by RefSeq, Jul 2008]

Function: Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Ref.11 Ref.20. Subunit structure: Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Interacts with CSPG4, FGFBP1 and TEC. Found in a complex with FGFBP1, FGF1 and FGF2. Ref.19 Ref.20 Ref.21 Ref.22 Ref.23 Ref.24 Ref.25. Subcellular location: Secreted. Nucleus. Note: Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57. Ref.25 Ref.28. Tissue specificity: Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue. Ref.11 Ref.18. Post-translational modification: Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion.Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing. Miscellaneous: This protein binds heparin more strongly than does aFGF. Sequence similarities: Belongs to the heparin-binding growth factors family. Biophysicochemical propertiespH dependence:Retains almost half of its activity after treatment at pH 2.0 for 3 hours at 20 degrees Celsius. Ref.11Temperature dependence:Inactivated after 3 minutes at 60 degrees Celsius or 1 minute at 80 degrees Celsius. Sequence caution: The sequence AAA52448.1 differs from that shown. Reason: Frameshift at positions 25, 82, 98 and 133. The sequence AAB21432.2 differs from that shown. Reason: Frameshift at position 25. The sequence AAB21432.2 differs from that shown. Reason: Unusual initiator. The initiator methionine is coded by a non-canonical CTG leucine codon.The sequence ABO43041.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence ABO43041.1 differs from that shown. Reason: Unusual initiator. The initiator methionine is coded by a non-canonical CTG leucine codon.The sequence CAA28027.1 differs from that shown. Reason: Frameshift at positions 25 and 102. The sequence CAA28027.1 differs from that shown. Reason: Unusual initiator. The initiator methionine is coded by a non-canonical CTG leucine codon.The sequence CAA73868.1 differs from that shown. Reason: Frameshift at position 25. The sequence CAA73868.1 differs from that shown. Reason: Unusual initiator. The initiator methionine is coded by a non-canonical CTG leucine codon.The sequence EAX05222.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence EAX05222.1 differs from that shown. Reason: Unusual initiator. The initiator methionine is coded by a non-canonical CTG leucine codon.

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