protein

Human CYR61

MBS691555

0.02 mg

340 USD

Recombinant Protein

Human CYR61

CYR61

Recombinant Human CYR61

protein CYR61; Protein CYR61; protein CYR61; IBP-10; IGFBP-10; CCN family member 1; IGF-binding protein 10; cysteine-rich, anigogenic inducer, 61; cysteine-rich heparin-binding protein 61; insulin-like growth factor-binding protein 10; cysteine-rich, angiogenic inducer, 61; CCN family member 1; Cysteine-rich angiogenic inducer 61; Insulin-like growth factor-binding protein 10; IBP-10; IGF-binding protein 10; IGFBP-10; Protein GIG1

CYR61

CYR61; CYR61; CCN1; GIG1; IGFBP10; CCN1; GIG1; IGFBP10; IBP-10; IGF-binding protein 10; IGFBP-10

CYR61_HUMAN

E Coli

Human

381

MTCPAACHCP LEAPKCAPGV GLVRDGCGCC KVCAKQLNED CSKTQP CDHT KGLECNFGAS STALKGICRA QSEGRPCEYN SRIYQNGESF Q PNCKHQCTC IDGAVGCIPL CPQELSLPNL GCPNPRLVKV TGQCCEE WVC DEDSIKDPME DQDGLLGKEL GFDASEVELT RNNELIAVGK GS SLKRLPVF GMEPRILYNP LQGQKCIVQT TSWSQCSKTC GTGISTRV TN DNPECRLVKE TRICEVRPCG QPVYSSLKKG KKCSKTKKSP EPV RFTYAGC LSVKKYRPKY CGSCVDGRCC TPQLTRTVKM RFRCEDGET F SKNVMMIQSC KCNYNCPHAN EAAFPFYRLF NDIHKFRD

> 95% by SDS-PAGE and HPLC analysis

Lyophilized from 10 mM Sodium Citrate, pH 3.0 + 50mM NaCl

The lyophilized protein is stable at room temperature for up to 1 month. Working aliquots stored with a carrier protein are stable for at least 5 months at -20 degree C to -80 degree C. Avoid repeated freeze thaw cycles.

Endotoxin Level: < 0.1 ng per ug of CYR61

Biological Activity: Determined by the dose-dependent stimulation of the proliferation of murine 3T3 cells. The expected ED50 for this effect is 2.0-3.0 ug/ml. Reconstitution: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8 degree C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20 degree C to -80 degree C.

Cytokines & Growth Factors

CYR61 is a member of the CCN family of secreted cysteine rich regulatory proteins. CYR61 induces angiogenesis by stimulating the proliferation, migration, and adhesion of endothelial cells. Cell migration and adhesion are mediated through binding to specific cell surface integrins and to heparin sulfate proteoglycans. Increased expression of CYR61 is associated with several types of cancer, and correlates with the progression and estrogen independence of human breast cancers. Recombinant human CYR61 is a 39.5 kDa protein containing 357 amino acid residues. It is composed of four distinct structural domains (modules); the IGF binding protein (IGFBP) domain, the von Willebrand Factor C (VWFC) domain, the Thrombospondin type-I (TSP type-1) domain, and a C-terminal cysteine knot-like domain (CTCK).

31542331

NP_001545.2

NM_001554.4

O00622

39,5 kDa

Hypertrophy Model Pathway (198828); Oncostatin M Signaling Pathway (711361); Regulation Of Wnt-mediated Beta Catenin Signaling And Target Gene Transcription Pathway (169352); RhoA Signaling Pathway (169356); Amb2 Integrin Signaling Pathway (137945)

The secreted protein encoded by this gene is growth factor-inducible and promotes the adhesion of endothelial cells. The encoded protein interacts with several integrins and with heparan sulfate proteoglycan. This protein also plays a role in cell proliferation, differentiation, angiogenesis, apoptosis, and extracellular matrix formation. [provided by RefSeq, Sep 2011]

Function: Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CYR61-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3. Ref.10. Subunit structure: Interaction with integrins is heparin- and cell-type-dependent and promotes cell adhesion. In skin fibroblasts it binds ITGA6/ITGB1, in endothelial cells, binds ITGAV/ITGB3 and in platelets, ITGA2B/ITGB3. Binds, in vitro, ITGAV/ITGB5. Subcellular location: Secreted. Sequence similarities: Belongs to the CCN family.Contains 1 CTCK (C-terminal cystine knot-like) domain.Contains 1 IGFBP N-terminal domain.Contains 1 TSP type-1 domain.Contains 1 VWFC domain.

0.02 mg

340 USD