protein

Human Galectin-3

MBS691554

0.05 mg

340 USD

Recombinant Protein

Human Galectin-3

Galectin-3

Recombinant Human Galectin-3

galectin-3 isoform 1; Galectin-3; galectin-3; lectin L-29; 35 kDa lectin; MAC-2 antigen; IgE-binding protein; laminin-binding protein; galactose-specific lectin 3; carbohydrate-binding protein 35; lectin, galactoside-binding, soluble, 3; 35 kDa lectin; Carbohydrate-binding protein 35; CBP 35; Galactose-specific lectin 3; Galactoside-binding protein; GALBP; IgE-binding protein; L-31; Laminin-binding protein; Lectin L-29; Mac-2 antigen

LGALS3; LGALS3; L31; GAL3; MAC2; CBP35; GALBP; GALIG; LGALS2; MAC2; Gal-3; CBP 35; GALBP

LEG3_HUMAN

E Coli

Human

250

ADNFSLHDAL SGSGNPNPQG WPGAWGNQPA GAGGYPGASY PGAYPG QAPP GAYPGQAPPG AYHGAPGAYP GAPAPGVYPG PPSGPGAYPS S GQPSAPGAY PATGPYGAPA GPLIVPYNLP LPGGVVPRML ITILGTV KPN ANRIALDFQR GNDVAFHFNP RFNENNRRVI VCNTKLDNNW GR EERQSVFP FESGKPFKIQ VLVEPDHFKV AVNDAHLLQY NHRVKKLN EI SKLGISGDID LTSASYTMI

> 98% by SDS-PAGE and HPLC analysis

Lyophilized from 0.5x PBS, pH 7.2

The lyophilized protein is stable for a 1 month at room temperature or 6 months at 4 degree C. The reconstituted protein is stable for 1 week at 2 degree C to 8 degree C and 3 months at -20 degreeC to 8 degree c and 3 months at -20 degree C to -80 degree C. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (e.g. 0.1% BSA) and store in working aliquots at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Endotoxin Level: < 0.1 ng per ug of Galectin-3

Biological Activity: Determined by its ability to chemoattract human blood monocytes. Chemotactic activity was observed at a concentration of 2.5 ug/ml with a peak response obtained at 250 ug/ml. Reconstitution: The human Galectin-3 should be reconstituted in water to a concentration of 0.1-1.0 mg/ml. Do not vortex! This solution can be diluted in water or other buffer solutions or stored at -20 degree C.

Cytokines & Growth Factors

Lectins, of either plant or animal origin, are carbohydrate binding proteins that interact with glycoprotein and glycolipids on the surface of animal cells. The Galectins are lectins that recognize and interact with beta-galactoside moieties. Galectin-3 regulates a number of biological processes, including embryogenesis, inflammatory responses, cell progression and metastasis. Galectin-3 is normally expressed in epithelia of a variety of tissues, including colon and endometrium, and in various inflammatory cells, including macrophages. Galectin-3 can function intracellularly, in controlling cell cycle and preventing T-cell apoptosis, and also extracellularly, in activating various cells, including monocytes/macrophages, mast cells, neutrophils, and lymphocytes. Expression of Galectin-3 is affected by neoplastic transformation, being up-regulated in certain types of lymphomas, and in thyroid and hepatic carcinomas. Conversely, it is down-regulated in other cancers, such as colon, breast, ovarian, and uterine. Recombinant human Galectin-3 is a globular 26.0 kDa protein containing 250 amino acid residues, but no disulfide bonds.

115430223

NP_002297.2

NM_002306.3

P17931

26.0 kDa

AGE/RAGE Pathway (698754); Advanced Glycosylation Endproduct Receptor Signaling Pathway (187092); Hedgehog Signaling Events Mediated By Gli Proteins Pathway (137912); Immune System Pathway (106386); Innate Immune System Pathway (106387); Spinal Cord Injury Pathway (739007)

This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Apr 2010]

Function: Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis . By similarity. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. Ref.15 Ref.16 Ref.18. Subunit structure: Probably forms homo- or heterodimers. Interacts with DMBT1 . By similarity. Forms a complex with the ITGA3, ITGB1 and CSPG4. Interacts with LGALS3BP, LYPD3, CYHR1 and UACA. Ref.14 Ref.15. Subcellular location: Cytoplasm. Nucleus. Secreted. Note: Secreted by a non-classical secretory pathway and associates with the cell surface. Ref.5 Ref.15 Ref.18. Tissue specificity: A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages. Expressed in fetal membranes. Ref.17. Sequence similarities: Contains 1 galectin domain.

0.05 mg

340 USD