Rabbit Anti-Human BD-4 | MyBioSource MBS691139 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit Anti-Human BD-4

catalog :

MBS691139

quantity :

0.1 mg

price :

415 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS691139

products type :

Antibody

products full name :

Rabbit Anti-Human BD-4

products short name :

[BD-4]

products name syn :

[DEFB104A; BD-4; DEFB4; hBD-4; DEFB-4; DEFB104; DEFB104B]

other names :

[beta-defensin 104; Beta-defensin 104; beta-defensin 104; beta-defensin 4; defensin, beta 104; defensin, beta 104B; Beta-defensin 4; BD-4; DEFB-4; hBD-4; Defensin, beta 104]

products gene name :

[BD-4]

other gene names :

[DEFB104B; DEFB104A; BD-4; hBD-4; DEFB-4; DEFB104; DEFB4; BD-4; DEFB-4; hBD-4]

uniprot entry name :

D104A_HUMAN

clonality :

Polyclonal

isotype :

Rabbit IgG

host :

Rabbit

reactivity :

Human

purity :

Antigen-affinity purified

form :

Lyophilized from PBS

storage stability :

The lyophilized antibody is stable for at least 5 years from date of receipt at -20°C to -80°C . The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C to -80°C. Avoid repeated freeze and thaw cycles.

tested application :

ELISA (EIA), Western Blot (WB)

app notes :

Western Blot: To detect hBD-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 uqg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBD-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Sandwich ELISA: To detect hBD-4 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with an appropriate secondary conjugated antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBD-4. OPTIMAL DILUTIONS SHOULD BE DETERMINED BY EACH LABORATORY FOR EACH APPLICATION!

other info1 :

Immunogen: E.coli derived Recombinant Human BD-4. Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hBD-4 (human Beta Defensin-4). Anti-Human BD-4 specific antibody was purified by affinity chromatography employing immobilized hBD-4 matrix.

other info2 :

Reconstitution Buffer: Water. Reconstitution: Reconstitute the antibody in sterile water to a concentration of 0.1 - 1.0 mg/ml.

products description :

Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The alpha-defensins are distinguished from the beta-defensins by the pairing of their three disulfide bonds. To date, six human beta-defensins have been identified; BD-1, BD-2, BD-3, BD-4, BD-5 and BD-6. beta-defensins are expressed on some leukocytes and at epithelial surfaces. In addition to their direct antimicrobial activities, they can act as chemoattractants towards immature dendritic cells and memory T cells. The beta-defensin proteins are expressed as the C-terminal portion of precursors and are released by proteolytic cleavage of a signal sequence and in some cases, a propeptide sequence. beta-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. BD-4 is expressed in testis, stomach, uterus, neutrophils, thyroid, lung and kidney. In addition to its direct antimicrobial activities, BD-4 is chemoattractant towards human blood monocytes.

ncbi gi num :

103485137

ncbi acc num :

NM_525128.2

ncbi gb acc num :

NM_001040702.1

uniprot acc num :

Q8WTQ1

ncbi pathways :

Beta Defensins Pathway (530759); Defensins Pathway (530757); Immune System Pathway (106386); Innate Immune System Pathway (106387)

ncbi summary :

Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Defensins are short, processed peptide molecules that are classified by structure into three groups: alpha-defensins, beta-defensins and theta-defensins. All beta-defensin genes are densely clustered in four to five syntenic chromosomal regions. Chromosome 8p23 contains at least two copies of the duplicated beta-defensin cluster. This duplication results in two identical copies of defensin, beta 104, DEFB104A and DEFB104B, in head-to-head orientation. This gene, DEFB104B, represents the more telomeric copy. [provided by RefSeq, Jul 2008]

uniprot summary :

Function: Has antimicrobial activity. Synergistic effects with lysozyme and DEFB103. Ref.1. Subcellular location: Secreted. Tissue specificity: High expression in the testis. Gastric antrum exhibited relatively high levels. A lower expression is observed in uterus and neutrophils thyroid gland, lung, and kidney. No detectable expression in other tissues tested. Ref.1. Induction: Antimicrobial activity is decreased when the sodium chloride concentration is increased. Sequence similarities: Belongs to the beta-defensin family.

size1 :

0.1 mg

price1 :

415 USD

more info or order :

MyBioSource product webpage