Rabbit Anti-Human PF-4 | MyBioSource MBS691129 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit Anti-Human PF-4

catalog :

MBS691129

quantity :

0.1 mg

price :

415 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS691129

products type :

Antibody

products full name :

Rabbit Anti-Human PF-4

products short name :

[PF-4]

products name syn :

[Antigen-Affinity Purified Polyclonal Antibodies Anti-human PF-4]

other names :

[platelet factor 4; Platelet factor 4; platelet factor 4; iroplact; oncostatin-A; C-X-C motif chemokine 4; chemokine (C-X-C motif) ligand 4; platelet factor 4; C-X-C motif chemokine 4; Iroplact; Oncostatin-A]

products gene name :

[PF-4]

other gene names :

[PF4; PF4; PF-4; CXCL4; SCYB4; CXCL4; SCYB4; PF-4]

uniprot entry name :

PLF4_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human

sequence length :

101

purity :

Antigen-affinity purified.

form :

Lyophilized from PBS

storage stability :

The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C.

tested application :

ELISA (EIA), Western Blot (WB), Neutr

app notes :

Sandwich ELISA: To detect human PF-4 by Sandwich ELISA (using 100 ul/well) a concentration of 0.5–2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 -0.4 ng/well of recombinant human PF- 4. Western Blot: To detect human PF - 4 by Western Blot analysis this antibody can be used at a concentra tion of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPF-4 is 1.5 -3.0 ng/lane, under either reducing or non-reducing conditions. Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human breast malignant ductal adenocarcinoma. The recommended concentration is 1.0 mg/mL -2.0 mg/mL with an overnight incubation at 4°C. An alkaline phosphatase-labeled polymer detection system was used with a red chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.

other info1 :

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant human PF 4 (human Platelet Factor-4). Anti-human PF 4 specific antibody was purified by affinity chromatography employing immobilized human PF 4 matrix. Immunogen: Recombinant Human PF-4. Reconstitution: Reconstitute the antibody in sterile water to a concentration of 0.1 - 1.0mg/ml . Reconstitution buffer: Water.

other info2 :

Buffer: PBS; pH 7.2

products description :

PF-4 is a CXC chemokine that is expressed in megakaryocytes and stored in the alpha-granules of platelets. PF-4 is chemotactic towards neutrophils and monocytes and has been shown to inhibit angiogenesis. Recombinant human PF-4 is a 7.8 kDa protein containing 70 amino acid residues, including the four highly conserved residues present in CXC chemokines.

ncbi gi num :

4505733

ncbi acc num :

NP_002610.1

ncbi gb acc num :

NM_002619.3

uniprot acc num :

P02776

ncbi pathways :

CXCR3-mediated Signaling Events Pathway (138011); Cell Surface Interactions At The Vascular Wall Pathway (106062); Chemokine Receptors Bind Chemokines Pathway (106359); Chemokine Signaling Pathway (99051); Chemokine Signaling Pathway (96864); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Common Pathway (106060); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Formation Of Fibrin Clot (Clotting Cascade) Pathway (106057)

ncbi summary :

This gene encodes a member of the CXC chemokine family. This chemokine is released from the alpha granules of activated platelets in the form of a homotetramer which has high affinity for heparin and is involved in platelet aggregation. This protein is chemotactic for numerous other cell type and also functions as an inhibitor of hematopoiesis, angiogenesis and T-cell function. [provided by RefSeq, Mar 2012]

uniprot summary :

Function: Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form. Ref.11. Subunit structure: Homotetramer. Ref.13 Ref.14. Subcellular location: Secreted. Sequence similarities: Belongs to the intercrine alpha (chemokine CxC) family. Mass spectrometry: Molecular mass is 7765 Da from positions 32 - 101. Determined by ESI. Ref.11Molecular mass is 6033 Da from positions 48 - 101. Determined by ESI. Short form. Ref.11

size1 :

0.1 mg

price1 :

415 USD

more info or order :

MyBioSource product webpage