Rabbit Anti-Human Fractalkine | MyBioSource MBS691049 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit Anti-Human Fractalkine

catalog :

MBS691049

quantity :

0.1 mg

price :

415 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS691049

products type :

Antibody

products full name :

Rabbit Anti-Human Fractalkine

products short name :

Fractalkine

products name syn :

Antigen-Affinity Purified Polyclonal Antibodies Anti-human Fractalkine

other names :

fractalkine; Fractalkine; fractalkine; C-X3-C motif chemokine 1; small-inducible cytokine D1; CX3C membrane-anchored chemokine; small inducible cytokine subfamily D (Cys-X3-Cys), member-1; small inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine, neurotactin); chemokine (C-X3-C motif) ligand 1; C-X3-C motif chemokine 1; CX3C membrane-anchored chemokine; Neurotactin; Small-inducible cytokine D1

other gene names :

CX3CL1; CX3CL1; NTN; NTT; CXC3; CXC3C; SCYD1; ABCD-3; C3Xkine; fractalkine; neurotactin; FKN; NTT; SCYD1

uniprot entry name :

X3CL1_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human

sequence length :

397

purity :

Antigen-affinity purified

form :

Lyophilized from PBS

storage stability :

The lyophilized antibody is stable for at least 2 years from date of receipt at -20 degree C. The reconstituted antibody is stable for at least two weeks at 2-8 degree C. Frozen aliquots are stable for at least 6 months when stored at -20 degree C. Avoid Repeated Freeze and Thaw Cycle!

tested application :

ELISA (EIA), Western Blot (WB)

app notes :

Western Blot: To detect human Fractalkine by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant human Fractalkine is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. ELISA: To detect human Fractalkine by direct ELISA (using 100ul/well antibody solution) a concentration of at least 0.5 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant human Fractalkine.

other info1 :

Antibody Generation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant human Fractalkine. Anti-human Fractalkine specific antibody was purified by affinity chromatography employing immobilized human Fractalkine matrix. Immunogen: Recombinant human Fractalkine. Reconstitution buffer: Water. Reconstitution: Reconstitute the antibody in sterile water to a concentration of 0.1-1.0mg/ml

other info2 :

Buffer: PBS; pH 7.2

products description :

CX3CL1, also known as Fractalkine, is a type I membrane protein in which a chemokine domain possessing a unique CX3C cysteine motif is tethered on a long mucin-like stalk. It can also be released as a soluble molecule upon proteolysis at a membrane proximal site.

ncbi gi num :

4506857

ncbi acc num :

NP_002987.1

ncbi gb acc num :

NM_002996.3

uniprot acc num :

P78423

ncbi mol weight :

42,203 Da

ncbi pathways :

Chemokine Receptors Bind Chemokines Pathway (106359); Chemokine Signaling Pathway (99051); Chemokine Signaling Pathway (96864); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Direct P53 Effectors Pathway (137939); GPCR Ligand Binding Pathway (161020); Peptide Ligand-binding Receptors Pathway (106358); Signal Transduction Pathway (477114)

uniprot summary :

Function: The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium. Binds to CX3CR1. Ref.8. Subunit structure: Monomer. Interacts with pox virus crmD; this inhibits cell migration mediated by CX3CL1. Ref.6 Ref.8. Subcellular location: Cell membrane; Single-pass type I membrane protein. Processed fractalkine: Secreted. Tissue specificity: Small intestine, colon, testis, prostate, heart, brain, lung, skeletal muscle, kidney and pancreas. Induction: By TNF and IL1/interleukin-1 in pulmonary endothelial cells and umbilical vein endothelial cells. Post-translational modification: A soluble short 95 kDa form may be released by proteolytic cleavage from the long membrane-anchored form . By similarity.O-glycosylated with core 1 or possibly core 8 glycans. Ref.5. Sequence similarities: Belongs to the intercrine delta family.

size1 :

0.1 mg

price1 :

415 USD

more info or order :

MyBioSource product webpage