antibody

Rat Anti-Mouse RAGE

MBS690938

0.1 mg

475 USD

monoclonal

rat

nonconjugated

(2B65)

western blot, immunohistochemistry

Antibody

Rat Anti-Mouse RAGE

RAGE

Anti-mouse Receptor for Advanced Glycation End Products [RAGE] (#2B65)

advanced glycosylation end product-specific receptor isoform a; Advanced glycation end-products receptor; advanced glycosylation end product-specific receptor; advanced glycation end-products receptor; receptor for advanced glycosylation end products; receptor for advanced glycation end-products variant 20; advanced glycosylation end product-specific receptor

RAGE

Ager; Ager; RAGE; RAGE

O35444_MOUSE

Monoclonal

IgG2

(2B65)

Rat

Mouse

402

This antibody detects mouse RAGE in Western blotting.

Protein G chromatography

Lyophilized

Lyophilized samples are stable for 2 years from date of receipt when stored at -70 degree C. Reconstituted antibody can be aliquoted and stored frozen at < -20 degree C for at least for six months without detectable loss of activity.

Western Blot (WB), Immunohistochemistry (IHC) - Formalin

1. Western blot: (1:500-1000) . 2. IHC (Frozen): (1:50-100)

Antibody Generation: This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with purified mouse recombinant RAGE extracellular domain. The IgG2 fraction of the culture supernatant was purified by Protein A/G affinity chromatography. Antigen: Recombinant mouse RAGE extracellular domain

Reconstitution Buffer: PBS (sterile). Reconstitution: Reconstitute the antibody with 200 ul sterile PBS and the final concentration is 500 ug/ml.

Advanced glycation endproducts (AGE) are adducts formed by the nonenzymatic glycation or oxidation of macromolecules. AGE forms during aging and its formation is accelerated under pathophysiologic states such as diabetes, Alzheimer's disease, renal failure and immune/inflammatory disorders. Receptor for Advanced Glycation Endoproducts (RAGE), named for its ability to bind AGE, is a multiligand receptor belonging the immunoglobulin (Ig) superfamily. Besides AGE, RAGE binds amyloid betapeptide, S100/calgranulin family proteins, high mobility group B1 (HMGB1, also know as amphoterin) and leukocyte integrins. The mouse RAGE gene encodes a 403 amino acid (aa) residue type I transmembrane glycoprotein with a 22 aa signal peptide, a 319 aa extracellular domain containing a Ig-like V-type domain and two Ig-like Ce-type domains, a 21 aa transmembrane domain and a 41 aa cytoplasmic domain. The V type domain and the cytoplasmic domain are important for ligand binding and for intracellular signaling, respectively. Two alternative splice variants, lacking the Vtype domain or the cytoplasmic tail, are known. RAGE is highly expressed in the embryonic central nervous system. In adult tissues, RAGE is expressed at low levels in multiple tissues including endothelial and smooth muscle cells, mononuclear phagocytes, pericytes, microglia, neurons, cardiac myocytes, and hepatocytes. The expression of RAGE is upregulated upon ligand interaction. Depending on the cellular context and interacting ligand, RAGE activation can trigger differential signaling pathways that affect divergent pathways of gene expression. RAGE activation modulates varied essential cellular responses (including inflammation, immunity, proliferation, cellular adhesion, and migration) that contribute to cellular dysfunction associated with chronic diseases such as diabetes, cancer, amyloidoses, and immune or inflammatory disorders.

110225341

NP_031451.2

NM_007425.3

O35444

Activated TLR4 Signalling Pathway (926081); Advanced Glycosylation Endproduct Receptor Signaling Pathway (926102); Cytosolic Sensors Of Pathogen-associated DNA Pathway (926112); DEx/H-box Helicases Activate Type I IFN And Inflammatory Cytokines Production Pathway (926120); Immune System Pathway (926012); Innate Immune System Pathway (926045); MyD88 Cascade Initiated On Plasma Membrane Pathway (926049); MyD88 Dependent Cascade Initiated On Endosome Pathway (926073); MyD88-independent Cascade Pathway (926065); MyD88:Mal Cascade Initiated On Plasma Membrane Pathway (926082)

RAGE: Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF- alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space. Interacts with S100B, S100A1 and APP. Interacts with S100A12. Endothelial cells. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Receptor, misc.; Cell cycle regulation; Motility/polarity/chemotaxis. Cellular Component: extracellular space; cell surface; membrane; cell soma; axon; cytoplasm; integral to membrane; plasma membrane; basal plasma membrane; cytosol. Molecular Function: identical protein binding; protein binding; advanced glycation end-product receptor activity. Biological Process: positive regulation of JNK activity; positive regulation of apoptosis; positive regulation of smooth muscle cell proliferation; negative regulation of collagen biosynthetic process; negative regulation of cell adhesion; positive regulation of smooth muscle cell migration; JAK-STAT cascade; induction of positive chemotaxis; activation of NF-kappaB transcription factor; calcium ion homeostasis; positive regulation of fibroblast proliferation; negative regulation of protein amino acid phosphorylation; regulation of DNA binding; positive regulation of neuron apoptosis; negative regulation of endothelial cell proliferation; regulation of inflammatory response; response to hypoxia; positive regulation of protein amino acid phosphorylation; inflammatory response; positive regulation of phagocytosis, engulfment; neurite development; positive regulation of autophagy; positive regulation of cell migration; positive regulation of inflammatory response

0.1 mg

475 USD